通过ATG8基化介导的质体阴道化减轻了真空球损伤
Xuanang Zheng1, Juncai Ma2, Jing Li2
1Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences; MOE Key Laboratory & Guangdong Provincial Key Laboratory of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou, China.
Nature communications
|July 18, 2025
概括
ATG8化迅速向质体,诱导真空膜浸泡和囊泡形成. 这个过程有助于真空酸化和植物适应性压力.
科学领域:
- 植物细胞生物学 植物细胞生物学
- 自学研究 自学研究
- 有机体动力学 有机体动力学
背景情况:
- ATG8化,ATG8家族蛋白质的脂化,主要以其在自细胞形成中的作用而闻名.
- 新兴证据表明,ATG8化在单膜细胞器中具有非正规的作用,但它们的功能意义尚不清楚.
研究的目的:
- 研究ATG8化在真空膜 (tonoplast) 上的非正规功能.
- 为了阐明ATG8ylation在真空重塑中的机制和功能后果.
- 确定ATG8化在植物适应环境压力的作用.
主要方法:
- 利用离子体 (单酶) 诱导ATG8对细胞的结合.
- 研究了活性氧物种 (ROS) 和V-ATPase在ATG8向中的参与.
- 评估了ATG8化对真空膜浸泡和内囊泡形成的影响.
- 研究了ATG8化在植物从压力中恢复和适应性条件中的作用.
主要成果:
- 离子体通过依赖ROS和V-ATPase的ATG8结合系统快速触发ATG8对质体的结合.
- ATG8附着在质体上促进了真空膜浸泡和内囊泡的形成,独立于ESCRT或细胞骨.
- ATG8化促进真空酸化恢复和植物生长恢复后单治疗.
- 在性压力下,ATG8ylation诱导类似的真空重塑,这表明了适应机制.
结论:
- ATG8化是真空膜重塑的关键调节器,与其在自中所扮演的正规角色不同.
- 这一过程增强了真空酸性,并促进了植物对性压力等环境挑战的弹性.
- 通过ATG8基化介导的真空重塑代表了植物中一种新的适应性策略.
相关概念视频
01:30ATP Driven Pumps III: V-type Pumps
4.0K
V-type pumps are ATP-driven pumps found in the vacuolar membranes of plants, yeast, endosomal and lysosomal membranes of animal cells, plasma membranes of a few specialized eukaryotic cells, and some prokaryotes. They are also known as the V1Vo-ATPase, that couple ATP hydrolysis to transport protons against a concentration gradient.
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
The peripheral or cytosolic V1 domain with eight subunits is involved in ATP hydrolysis. The integral or transmembrane V0 domain containing at least five subunits...
4.0K
01:11Protein Transport to the Outer Chloroplast Membrane
2.0K
Chloroplast outer membrane proteins encoded by the nucleus are synthesized in the cytosol. Soon after synthesis, they bind cytosolic factors such as 14-3-3 protein and the Hsp70 chaperones that keep these precursors in an unfolded state until their translocation.
Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.
Two models describe the mechanism of precursor recognition and entry across the outer membrane through the TOC complex. Model 1 suggests the newly synthesized precursor binds to the TOC receptor 159 and forms a complex.
2.0K
01:46The Apoplast and Symplast
51.5K
Plant growth depends on its ability to take up water and dissolved minerals from the soil. The root system of every plant is equipped with the necessary tissues to facilitate the entry of water and solutes. The plant tissues involved in the transport of water and minerals have two major compartments - the apoplast and the symplast. The apoplast includes everything outside the plasma membrane of living cells and consists of cell walls, extracellular spaces, xylem, phloem, and tracheids. The...
51.5K
01:18Protein Transport to the Inner Chloroplast Membrane
2.1K
Proteins targeted to the inner chloroplast membrane, or plastid proteins, are transported by two general pathways: the stop-transfer and the re-insertion or post-import pathways. Most plastid proteins carry N-terminal transit sequences and internal import sequences targeting it to the specific chloroplast subcompartment. Proteins targeted by the stop-transfer pathway have internal hydrophobic sequences that inhibit their translocation into the stroma. As a result, these precursors are arrested...
2.1K
00:53Tonicity in Plants
54.7K
Tonicity describes the capacity of a cell to lose or gain water. It depends on the quantity of solute that does not penetrate the membrane. Tonicity delimits the magnitude and direction of osmosis and results in three possible scenarios that alter the volume of a cell: hypertonicity, hypotonicity, and isotonicity. Due to differences in structure and physiology, tonicity of plant cells is different from that of animal cells in some scenarios.
54.7K
01:32Pinching-off of Coated Vesicles
3.2K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
3.2K