相关实验视频

Updated: Sep 14, 2025

Identification of Protein Interacting Partners Using Tandem Affinity Purification
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Identification of Protein Interacting Partners Using Tandem Affinity Purification

Published on: February 25, 2012

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使用小麦胚芽聚氨酸沉物分离培养哺乳动物细胞和组织样本中的糖蛋白的协议

Simran Chopra1, Otsuware Linda-Josephine Kadiri1, Kai Kappert2

  • 1Department of Molecular and Experimental Nutritional Medicine, Institute of Nutritional Science, University of Potsdam, Arthur-Scheunert-Allee 114-116, 14558 Nuthetal, Germany.

STAR protocols
|July 19, 2025
PubMed
概括

这项研究详细介绍了使用小麦胚芽聚合素 (WGA) 沉来分离糖蛋白的协议. 该方法从细胞和组织样本中丰富特定的蛋白质,如密度增强酸酶 (DEP-1).

关键词:
细胞生物学 细胞生物学代谢过程中的代谢.分子生物学分子生物学

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相关概念视频

Immunoprecipitation01:20

Immunoprecipitation

Immunoprecipitation, or IP, is a widely used technique that employs protein-antibody interactions to isolate proteins or protein complexes in their native state for studying protein-protein interactions, quaternary structures, or supramolecular complexes. Various modifications of the technique, including chromatin IP, cross-linking IP, and fluorescence IP, are commonly used.
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