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相关概念视频

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Ribosome Profiling02:24

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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相关实验视频

Updated: Sep 14, 2025

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
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使用scComplete-seq的单细胞总转录组的自动化高通量分析.

Fatma Betül Dinçaslan1,2, Shaun Wei Yang Ngang1,2, Rui Zhen Tan3

  • 1Department of Biomedical Engineering, Faculty of Engineering, National University of Singapore, Singapore 117583, Singapore.

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概括

我们开发了scComplete-seq,这是一种新的单细胞测序方法,可以检测到多基和非多基RNA. 这一突破揭示了非多基核糖核酸,对于理解细胞功能至关重要.

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科学领域:

  • 分子生物学分子生物学
  • 基因组学就是基因组学.
  • 一个单细胞分析.

背景情况:

  • 了解完整的转录组,包括多基和非多基RNA,对于阐明细胞功能至关重要.
  • 目前的单细胞分析方法在全面评估总细胞转录组时面临局限性,原因是缺乏自动化,高吞吐量测试.

研究的目的:

  • 开发和验证 scComplete-seq,一种增强的基于滴滴的单细胞测序方法.
  • 为了在单细胞分辨率下使非多聚烯化转录组的高通量检测成为可能.

主要方法:

  • 增强现有的基于滴滴的单细胞mRNA测序.
  • 应用scComplete-seq来检测长短的非多基尼化RNAs.
  • 对癌细胞和外围血液单核细胞 (PBMCs) 的转录组变化的分析.

主要成果:

  • scComplete-seq在单细胞中成功检测出非多基尼化RNA,包括基因组RNA和增强型RNA.
  • 该方法提供单细胞分辨率,用于分析非多聚烯酸转录基因组.
  • 在不同的刺激下确定了PBMCs的转录组变化,突出了特定的生物过程和增强剂活动.

结论:

  • scComplete-seq为全面的单细胞转录组分析提供了一个强大的工具.
  • 该方法有助于研究非多聚基核糖核酸,扩大我们对细胞生物学的理解.
  • scComplete-seq可以揭示动态的转录基因变化和调节机制,以响应细胞刺激.