perfluorodecanesulfonate (PFDS) 在生命早期斑马鱼中通过NF-κB通路诱导先天性免疫毒性

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Chengwei Zhou¹, Wei Chen¹, Rongrong Xuan²

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Environment international

|July 19, 2025

概括

在斑马鱼中暴露于 perfluorodecanesulfonate (PFDS) 通过激活NF-κB通路来破坏免疫功能. PFDS显示出比PFOS更强的免疫毒性,突出了水生环境中不受监管的PFAS的风险.

科学领域:

环境毒理学环境毒理学
水生物质毒理学研究
免疫毒理学免疫毒理学

背景情况:

长链多醇基物质 (PFAS),如 perfluorodecanesulfonate (PFDS),在水生环境中具有持久性和生物积累性.
PFDS在水生生物中的免疫毒性尚不清楚.
PFDS越来越多地被用作对 perfluorooctanesulfonate (PFOS) 的替代品.

研究的目的:

在生命早期的斑马鱼中描述PFDS的免疫毒性作用.
为了比较PFDS与PFOS在同等度下的免疫毒性.
阐明PFDS诱导的免疫毒性背后的分子机制.

主要方法:

斑马鱼 (Danio rerio) 幼虫在120小时内暴露在环境相关的PFDS和PFOS度中.
分析了免疫细胞群 (巨细胞,中性粒细胞),氧化应激标记物和免疫信号分子 (介质蛋白,免疫球蛋白).
核因子kappa B (NF-κB) 信号通路的作用被使用morpholino knockdown进行了研究.

主要成果:

在斑马鱼幼虫中生物积累的PFDS并显著破坏了免疫功能.
暴露改变了免疫细胞数量,诱导了氧化应激,以及失调的关键免疫标记物.
PFDS激活了NF-κB通路,导致促炎性细胞因子表达和免疫功能障碍.
与PFOS相比,PFDS显示出更强的免疫毒性作用.

结论:

在斑马鱼中,PFDS诱导显著的免疫毒性,通过NF-κB通路激活的介导.
由于其较强的不良影响,PFDS可能比PFOS带来更大的生态风险.
这些发现为在水生生态系统中对PFDS和其他不受监管的PFAS的生态风险评估提供了关键数据.

关键词:

生物积累生物积累生物积累鱼的生命早期阶段鱼的生命早期阶段免疫毒性反应的反应核因子卡帕B信号通路的核因子.在PFAS中,我们可以使用PFAS.

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相关概念视频

NF-κB-dependent Signaling Pathway

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...