来自Pericarpium Citri Reticulatae"Chachi"的生物活性成分通过PI3K/AKT信号通路缓解性结肠炎:网络药理学,分子对接和实验验证
Qian Li1, Shan-Shan Wu1, Bai-Zhong Chen2
1Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, School of Pharmacy and Food Engineering, Wuyi University, Jiangmen, China; International Healthcare Innovation Institute (Jiangmen), Jiangmen, China.
Fitoterapia
|July 19, 2025
概括
异素素 (ISS),是由Citri Reticulatae Pericarpium衍生而来的
科学领域:
- 药理学和中国传统医学
- 胃肠病学 胃肠病学
- 分子生物学分子生物学
背景情况:
- Reticulatae Pericarpium 'Chachi' (PCRC) 是一种传统的中国草药,已知对胃肠道疾病有好处.
- 性结肠炎 (UC) 是一种慢性炎症性肠病,治疗选择有限.
- 针对UC治疗的PCRC的特定生物活性化合物和分子机制在很大程度上仍未被阐明.
研究的目的:
- 为了确定PCRC中的有效抗UC的活性成分.
- 通过网络药理学和分子对接,探索PCRC治疗效果背后的分子机制.
- 在UC模型中实验验证已识别的化合物的疗效和机制.
主要方法:
- 高性能液体染色学-质谱学 (HPLC-MS) 用于组件识别.
- 网络药理学和分子对接用于机制预测.
- 在体外测定氧化 (NO) 和炎症因子释放.
- 使用UC小鼠模型进行体内研究,以评估症状缓解,氧化应激,炎症和肠道屏障完整性.
- 通过KEGG/GO的缩分析和Westernblotting来阐明信号通路.
- 16S rRNA测序以分析肠道微生物群的组成.
主要成果:
- 在PCRC中,HPLC-MS确定了40个成分;网络药理学和分子对接确定了异素素 (ISS) 为关键候选物.
- 在体外和体内,ISS表现出显著的抗炎和抗氧化作用.
- 在UC小鼠模型中,ISS治疗减少了疾病严重程度,氧化应激 (MDA,GSH,SOD) 和炎症标志物 (IL-6,IL-1β,TNF-α).
- ISS增强了肠上皮质屏障的完整性和调节了肠道微生物群的组成.
- 西部斑点分析证实ISS抑制PI3K/AKT信号通路.
结论:
- 异宁素 (ISS) 被确定为PCRC的主要生物活性化合物,对性结肠炎具有显著的治疗潜力.
- 通过减少炎症,氧化应激和改善肠道屏障功能,主要通过抑制PI3K/AKT通路来发挥其保护作用.
- ISS还证明了恢复UC诱导的肠道微生物群失调的能力,这表明其广泛的治疗作用和UC治疗中临床应用的潜力.
关键词:
树网状树 (Citri Reticulatae Pericarpium) 茶树 (Chachi) 树 (Citri Reticulatae Pericarpium) 树 (Chachi) 树 (Citri Reticulatae Pericarpium) 树 (Chachi) 树 (Citri Reticulatae Pericarpium) 树 (Citri Reticulatae Pericarpium) 树 (Citri Reticulatae Pericarpium) 树 (Chachi) 树 (Citri Reticulatae Pericarpium) 树 (Citri Reticulatae Pericarpium) 树 (Citri Reticulatae) 树 (Citri Reticulatae) 树 (Citri) 树 (Citri) 树 (Citri) 树 (Citri) 树 (Citri) 树 (Citri) 树 (Citri)我们的肠道微生物群.这种异素是异素.网络药理学 网络药理学性结肠炎是一种相关概念视频
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