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设计一种带有性能增强开关的大肠杆菌,利用CRISPR-Cas9系统作为生物膜形成抑制的活体定数灭器

Jinjie Zhou¹, Chenyang Zhou¹, Guanyu Jiang¹

¹School of Environmental Science and Engineering, Tianjin University, Tianjin, 300072, China; Tianjin Key Lab of Indoor Air Environmental Quality Control, Tianjin, 300072, China; State Key Laboratory of Synthetic Biology, Tianjin University, Tianjin, 300072, China.

Environmental research

|July 19, 2025

概括

带有新型遗传开关的工程细菌有效地杀了细菌的交流,显著减少了生物膜的形成. 这一策略为复杂系统中的生物膜控制提供了一种有希望的,环保的方法.

科学领域:

微生物学微生物学
基因工程是一种基因工程.
环境生物技术环境生物技术

背景情况:

质量灭 (QQ) 破坏了细菌的交流和生物膜的形成.
野生类型的QQ细菌具有有限的基因表达调节能力.
控制生物膜的形成在各种环境和工业环境中至关重要.

研究的目的:

用一种性能增强的开关来设计大肠杆菌,用于调制定数灭基因表达.
评估工程细菌 (EB) 在减少活性污泥系统中的生物膜形成方面的有效性.
评估转基因菌株对环境的影响.

主要方法:

利用CRISPR-Cas9系统将QQ基因 (aiiO) 和记者基因 (GFP) 整合到大肠杆菌中.
开发了一种基因开关,允许IPTG诱导性aiO的表达.
带有EB和量化生物膜参数的注射活性污泥系统.

主要成果:

经过IPTG诱导的工程细菌显示QQ活动增加了2.34倍.
在活性污泥系统中,EB减少了24.72%的生物膜形成和22.96%的生物膜厚度.
总微生物生物质 (57.68%),EPS分泌 (29.88%) 和粘附强度 (34.31%) 显著减少.

结论:

带有性能增强开关的工程细菌为生物膜控制提供了有效的策略.
基因开关在复杂的环境条件下表现出持续的功能.
开发的方法显示了最小的环境影响和适用于现实世界的系统.

关键词:

抑制生物膜形成抑制生物膜形成克里斯普尔-Cas9是什么意思基因表达调节基因表达调节微生物的基因编辑.议员数量减少的原因

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