相关实验视频

Updated: Sep 14, 2025

Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory
08:02

Benchtop Immobilized Metal Affinity Chromatography, Reconstitution and Assay of a Polyhistidine Tagged Metalloenzyme for the Undergraduate Laboratory

Published on: August 23, 2018

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对GMHpaB的生物化学和结构洞察:一种热稳定的4-Hydroxyphenylacetate-3-monooxygenase,具有双重辅助因子的多功能性

Nor Asyikin Che Husain1, Azyyati Mohd Padzil2, Azney Zuhaily Md Taib2

  • 1Department of Bioscience, Faculty of Science, Universiti Teknologi Malaysia, 81310 Johor Bahru, Johor, Malaysia; Structural Biology and Functional Omics, Malaysia Genome and Vaccine Institute, National Institutes of Biotechnology Malaysia, 43000 Kajang, Selangor, Malaysia.

International journal of biological macromolecules
|July 19, 2025
PubMed
概括

研究人员克隆和净化了GMHpaB,这是来自Geobacillus mahadii的热稳定氧酶,对4-基酸盐降解至关重要. 这种酶表现出高活性和双辅助因子兼容性,使潜在的高温生物催化剂应用成为可能.

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Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition08:31

Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition

Published on: October 3, 2018

8.6K
Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry12:08

Monitoring the Reductive and Oxidative Half-Reactions of a Flavin-Dependent Monooxygenase using Stopped-Flow Spectrophotometry

Published on: March 18, 2012

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相关概念视频

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Cofactors and Coenzymes

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Cofactors can be metallic ions or organic molecules called coenzymes. These types of helper...
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Cooperative Allosteric Transitions

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In the presence of oxidizing agents, phenols are oxidized to quinones. Quinones can be easily reduced back to phenols using mild reducing agents. The electron-donating hydroxyl group enhances the reactivity of the aromatic ring, enabling oxidation of the ring even in the absence of an α hydrogen.
o-hydroxy phenols are oxidized to o-quinones and p-hydroxy phenols to p-quinones. Such redox reactions involve the transfer of two electrons and two protons. The reversible redox...
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