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使用RPA-CRISPR在离心力驱动的微流体系统上进行沙门氏菌的视觉和独立诊断

Jun Chen¹, Yifan Lu¹, Xiaobo Meng¹

¹Key Laboratory for Food Microbial Technology of Zhejiang Province, School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, 310018, Zhejiang, China.

Food microbiology

|July 19, 2025

概括

本研究提出了一种使用CRISPR-Cas12a和微流体芯片上的重组酶聚合酶放大 (RPA) 快速检测食品中的沙门氏菌的新方法. 便携式系统在50分钟内提供灵敏的视觉检测,提高了食品安全.

科学领域:

食品安全和微生物学
分子诊断学分子诊断学
生物传感器技术的技术

背景情况:

快速和敏感的核酸检测对于识别食品中的沙门氏菌至关重要.
现有的CRISPR-Cas12a方法在灵敏度上有局限性,原因是单反应.
方便的检测方法对于改善食品安全监督至关重要.

研究的目的:

开发一个集成的微流体芯片来检测沙门氏菌.
为了克服传统CRISPR-Cas12a系统的局限性.
为食品安全创建一个便携式和可视检测的核酸测定.

主要方法:

在微流体芯片上集成CRISPR-Cas12a系统与重组酶聚合酶放大 (RPA).
开发一种便携式观测设备,用于肉眼检测信号.
反应条件的优化,以快速和敏感地识别沙门氏菌.

主要成果:

开发的技术专门检测到1 x 10°CFU/mL的沙门氏菌.
在37°C的温度下,在50分钟内实现了检测.
该系统在不需要专用设备的情况下成功进行了现场测试.

结论:

在微流体芯片上集成的CRISPR-Cas12a和RPA系统提供了一种敏感和快速的沙门氏菌检测方法.
这种便携式可视检测测试剂为食品安全应用提供了方便的解决方案.
该技术消除了交叉污染风险,并简化了现场使用的检测程序.

关键词:

这就是CRISPR/Cas12a.集成检测装置的综合检测装置微流体芯片是一个微流体.复合酶聚合酶放大复合酶的放大.沙门氏菌是一种沙门氏菌.

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