每日暴露于初级和新兴芳氨酸所引起的氧化损伤:从大规模生物监测和基于细胞的高通量PCR阵列分析中获得的见解
Shaohan Zhang1, Zijun Zhou1, Pingping Wang2
1MOE Key Laboratory of Pollution Processes and Environmental Criteria, College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China.
Journal of hazardous materials
|July 20, 2025
概括
这项研究监测了中国的芳氨酸暴露,发现了显著的区域差异,并将特定化学物质与氧化应激和DNA,RNA,蛋白质和脂质的损伤联系起来. 这些发现有助于优先考虑用于减轻健康风险的化学品.
科学领域:
- 环境健康科学 环境健康科学
- 毒理学 毒理学 毒理学
- 生物监测 生物监测
背景情况:
- 芳香胺 (AAs) 在工业上具有重要意义,但它们的住宅暴露和氧化损伤效应尚不清楚.
- 需要进行全面的生物监测和机械学研究,以描述来自AAs的氧化损伤.
- 这项研究的重点是中国人群中的初级芳氨酸 (PAA),新兴芳氨酸 (AA) 和它们的类衍生物 (PPD-Qs).
研究的目的:
- 评估在中国每天接触PAA,AA和PPD-Qs所引起的氧化损伤.
- 通过先进的统计分析来确定氧化应激的主要化学贡献者.
- 为了研究氧化损伤的体外机制,由优先的芳香氨基化学物质引起的.
主要方法:
- 对来自中国的397个尿样进行大规模生物监测,量化了11个PAA,20个AA和6个PPD-Qs.
- 先进的统计回归模型 (MLR,WQS,BKMR) 用于将化学物质暴露与氧化应激生物标志物 (8-OHdG,8-OHG,di-Y,MDA) 相对应.
- 在体外基于细胞的高通量PCR阵列分析以评估与氧化应激相关的活性氧物种 (ROS) 生产和基因表达.
主要成果:
- 尿液度显示 ΣPAAs > ΣAAs > ΣPPD-Qs,观察到显著的区域差异.
- 统计模型确定了与DNA,RNA,蛋白质和脂质损伤相关的关键化学物质 (例如DPG,TPG,DTG,DPPD-Q,CPPD-Q,2-NA).
- 实验室内实验证实,优先考虑的化学物质显著增加了ROS (118%-241%) 和与氧化压力相关的基因失调.
结论:
- 这项研究提供了对中国人群中芳胺诱导的氧化损伤的多方面的理解.
- 确定了导致氧化应激的关键化学物质,突出了与暴露相关的特定风险.
- 这些发现支持化学品优先级和监管策略,以减轻由芳胺暴露引起的健康风险.
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