标准化kombucha与量身定制的微生物联盟开发:化合物概况和抗氧化活性在体外和Caenorhabditis elegans
Bruna Krieger Vargas1, Mariana Fensterseifer Fabricio1, Luana Schmidt1
1Food Science and Technology Institute, Brazil.
Food chemistry
|July 20, 2025
概括
这项研究表明,绿茶kombucha富含化合物,提供抗氧化剂的好处,没有毒性. 每天摄入600毫升可能会提供可行的抗氧化剂保护.
科学领域:
- 食品科学 食品科学 食品科学
- 生物化学 生物化学
- 毒理学 毒理学 毒理学
背景情况:
- 康布查是一种发酵茶,以其丰富的含量而闻名.
- 化合物与各种生物活动有关,包括抗氧化特性.
- 标准化绿茶kombucha的特定成分和抗氧化作用需要详细的研究.
研究的目的:
- 为了表征标准化绿茶kombucha的性特征.
- 为了评估不同度的kombucha的体外和体内抗氧化活性.
- 评估kombucha在体外和体外模型中的潜在毒性.
主要方法:
- 使用二极管阵列检测和质谱学 (HPLC-DAD-MS/MS) 的高性能液体染色学用于化合物识别和量化.
- 进行了体外测试,以测量反应性物种和脂质过氧化的减少.
- 在体内研究中使用C. elegans (C. elegans) 来评估毒性和抗氧化剂反应,包括超氧化物失调酶 (SOD) 光和存活率.
主要成果:
- 在HPLC-DAD-MS/MS分析中,确定了35种化合物,重点是 (Epi) 甲基酸衍生物.
- 在25 ,125和250μL•mL-1的度下,康布查没有表现出体内毒性.
- 评估的kombucha剂量有效地减少了活性物种和脂质过氧化在体外,并增加了C. elegans的SOD光,尽管它们没有保护H2O2诱导的死亡率.
结论:
- 绿茶kombucha具有复杂的性特征,有助于其抗氧化特性.
- 被测试的kombucha度在体外和体内表现出抗氧化作用,而不会引起毒性.
- 建议每天摄入600毫升这种特定的kombucha作为抗氧化剂保护的可行量.
- 可能需要进一步的研究来增强对抗氧化应激诱导死亡的保护作用.
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