污水污泥的工业堆肥减轻了抗菌素耐药性的风险,并保持了热带土壤中的细菌动态
Rafael Santana Mendonça1, Adijailton José de Souza1, Rafael Marques Pereira Leal2
1Department of Soil Science, "Luiz de Queiroz" College of Agriculture (ESALQ), University of São Paulo (USP), Avenida Pádua Dias 11, PO box 9, Piracicaba, 13418-900, São Paulo, Brazil.
Journal of environmental management
|July 20, 2025
概括
污水污泥的工业堆肥有效地减少了土壤中的抗生素耐药性基因 (ARG). 这一过程减轻了抗菌素耐药性风险,尽管对热带农业生态系统的长期影响需要进一步调查.
科学领域:
- 环境微生物学环境微生物学
- 土壤科学 土壤科学
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 污水污泥 (SS) 堆肥是一种减少农业土壤中抗菌素耐药性 (AMR) 风险的潜在策略.
- 应用SS堆肥对土壤微生物群落和抗生素耐药性基因 (ARG) 的影响尚未完全理解.
研究的目的:
- 评估热友性堆肥的SS,有或没有石灰,对热带土壤中的ARG,移动遗传元素 (MGE) 和细菌群落的影响.
- 评估工业化堆肥在减轻农业生态系统中AMR风险方面的有效性.
主要方法:
- 使用高通量PCR (qPCR) 来量化ARG和MGE.
- 16S rRNA基因测序分析了细菌社区的组成.
- 在100天的时间里,在热带粘土和沙土壤中进行了实验.
主要成果:
- 工业堆肥显著减少了ARG,特别是硫胺耐药基因.
- 堆肥改变了细菌社区结构,Actinobacteriota的数量增加.
- 非石灰堆肥最初减少了微生物的多样性,而石灰提高了堆肥的稳定性.
结论:
- 工业规模的热友堆肥是一种可行的方法,可以减少热带农业土壤中AMR风险.
- 特定的细菌系被确定为ARG的潜在载体.
- 需要进一步进行长期研究,以了解热带野外条件下的ARG持久性和环境传播.
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