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Updated: Sep 14, 2025

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在撒丁岛绵羊奶酪加工环境中,用于识别和评估一般性和潜在病原性细菌的相对丰度的DNA元编码

L Giagnoni¹, S Salza², A Tondello³

¹Department of Veterinary Medicine, University of Sassari, Via Vienna 2, 07100 Sassari, Italy; Department of Agronomy, Food, Natural Resources, Animals and Environment, DAFNAE, University of Padova, Viale dell'Università 16, 35020 Legnaro, PD, Italy.

International journal of food microbiology

|July 20, 2025

概括

羊奶奶酪设施中的微生物污染是一个食品安全问题. 细菌元编码揭示了不同加工区域的独特微生物群落,其中Halomonas在某些区域占主导地位.

科学领域:

食品微生物学食品微生物学
环境微生物学环境微生物学
乳制品科学乳制品科学

背景情况:

微生物污染是乳制品加工的重大挑战,影响食品安全和质量.
严格的卫生协议是必不可少的,特别是在像撒丁岛绵羊奶酪生产这样的专业环境中.
加工设施内的特定位置可以容纳腐烂和致病微生物,如Listeria monocytogenes,Salmonella spp.,Bacillus cereus和Pseudomonas spp.

研究的目的:

用基于16S rRNA的metabarcoding评估撒丁岛奶酪加工环境中的细菌种群.
为了获得有关整个微生物群的详细分类信息,包括潜在的致病菌株.
为了确定各种加工区域和表面类型的微生物群落差异.

主要方法:

基于16S rRNA的细菌元编码被用来分析细菌群落.
在撒丁岛的14家乳制品工厂的9个地区,从253个地点采集了样本.
生物信息处理超过5100万个高质量的过阅读,确定了4426个分类系.

主要成果:

在洗加工区域和盐老化架空区域之间观察到细菌群体组成的明确划分.
哈洛蒙纳斯属和哈洛蒙纳科家族被确定为主导和差异化的种类.
潜在病原体的序列主要在与盐适应生物群占主导地位的区域不同的地方发现.

结论:

细菌元编码有效地描述了乳制品加工环境中的微生物群落.
处理工作流程阶段显著影响微生物社区结构,在不同区域具有不同的微生物配置文件.
了解这些微生物动态对于实施有针对性的卫生战略来确保奶酪生产中的食品安全至关重要.

关键词:

16S rRNA 的元编码.交叉污染是一种交叉污染.乳制品加工环境乳制品加工环境环境监测环境监测环境监测食品安全食品安全微生物污染的发生

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