使用Novacetimonas hansenii TGA隔离物从脱的米中可持续生产细菌纤维素:过程优化和膜表征
Ahmed K Saleh1, Hamada El-Gendi2, Gamal A G Ammar3
1Cellulose and Paper Department, National Research Centre, El-Tahrir St., Dokki 12622, Giza, Egypt.
International journal of biological macromolecules
|July 20, 2025
概括
酸/预处理有效地去除细菌纤维素 (BC) 生产的细菌纤维素. 这种优化的工艺从水解米中获得了高质量的BC,显示了生物材料的潜力.
科学领域:
- 生物技术是生物技术.
- 材料科学 材料科学 材料科学
- 可持续化学 可持续化学
背景情况:
- 米 (RS) 是一个丰富的农业废物,具有生物制品的潜力.
- 细菌纤维素 (BC) 是一种多功能生物材料,在医药和包装中具有应用.
- 效率高的纤维素生物质预处理和水解对于具有成本效益的生物加工至关重要.
研究的目的:
- 调查不同预处理方法对大米草脱的有效性.
- 为了优化酶解水解,从预处理的米中释放葡萄糖.
- 使用新型菌株和优化的发酵条件生产和表征细菌纤维素.
主要方法:
- 评估了酸/,热水和酸预处理,用于米的脱.
- 使用细胞酶进行预处理的米的酶性水解.
- 隔离并确定了一种生产纤维素的细菌菌株 (Novacetimonas hansenii TGA).
- 优化BC生产的发酵介质和条件.
- 使用FT-IR和结晶性分析对BC结构和特性进行了表征.
主要成果:
- 酸/预处理实现了76.6%的脱,显著提高了纤维素含量.
- 从酸/处理的米中获得了17.4 mg/mL的最大葡萄糖释放.
- 诺瓦塞蒂莫纳斯汉森尼 (Novacetimonas hansenii TGA) 从米水解液中产生了3.21g/L的BC.
- 与标准介质相比,在改性介质中生产的BC表现出更厚的纳米纤维和略有降低的晶度.
- FT-IR分析证实了纤维素结构,HS和R2介质产生稳定的BC.
结论:
- 酸/预处理对于大米草脱是非常有效的,可以有效地生产BC.
- 使用Novacetimonas hansenii TGA进行优化的酶解水解和发酵,为从农业废物中合成BC提供了一种经济有效的途径.
- 这项研究展示了可持续BC生产的新型综合方法,其在生物材料,包装和医药领域的潜在应用.
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