超排卵期间的孕激素度和雌性行为会影响荷尔斯坦公牛的雌性后子宫内膜基因表达
J C S Marques1, J P O Maciel2, J Denis-Robichaud1
1Faculty of Land and Food Systems, University of British Columbia, Vancouver V6T 1Z4, Canada.
Journal of dairy science
|July 20, 2025
概括
孕激素度和雌激素表达强度相互作用,改变子宫内膜基因表达在雌牛种植后. 雌激素在雌激素前的水平也会影响基因表达,影响免疫和生殖功能.
科学领域:
- 生殖生物学 生殖生物学
- 基因组学就是基因组学.
- 动物科学动物科学
背景情况:
- 超排卵协议旨在提高牛的生殖效率.
- 子宫内膜基因表达对于成功建立怀孕至关重要.
- 荷尔蒙特征,包括孕激素 (P4) 和雌激素 (E2),影响子宫的受容性.
研究的目的:
- 为了确定在超排卵期间的不同孕激素度和不同雌性表达强度是否会影响Holstein母牛在人工授精 (AI) 后7天内宫内基因表达.
- 调查前雌激素 (E2) 度与P4相关的作用,以及雌激素表达对子宫内膜基因表达的影响.
主要方法:
- 在超排卵期间,霍尔斯坦牛犊被分配到高或低P4治疗组.
- 通过自动传感器监测雌性行为;小母被按雌性表达强度分类.
- 用NanoString nCounter系统在子宫活检样本上对子宫内膜基因表达进行了分析,AI后7天.
主要成果:
- P4治疗和雌性表达强度之间的相互作用显著改变了12个子宫内膜基因的表达,主要与免疫信号相关.
- 低P4治疗与低雌性表达相结合,导致子宫内膜中免疫功能的上调.
- 化前的E2度独立地影响了参与细胞外矩阵重塑,免疫反应,生长因子和eicosanoid代谢的基因.
结论:
- 在超排卵期间接受低P4并表现出低雌性表达的母牛在AI后7天显示出明显的子宫内膜基因表达特征.
- 雌激前的E2度在调节雌激后子宫内膜基因表达方面发挥着重要作用,独立于雌激前的P4形状.
- 这些发现突出了激素和行为因素的复杂相互作用,影响子宫受体性和牛的潜在怀孕结果.
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