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Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
Published on: December 31, 2017
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非手术性牙周治疗调节了原发性免疫缺陷儿童的口腔微生物群
Abish S Stephen1,2, Stacy Worrall1,2, Cheryl Somani1,3
1Centre for Oral Immunobiology & Regenerative Medicine, Institute of Dentistry, Faculty of Medicine and Dentistry, Queen Mary University London, London, UK.
Journal of clinical periodontology
|July 20, 2025
概括
初级免疫缺陷 (PID) 影响口腔健康. 非手术性牙周治疗改变了PID儿童的 subgingival 微生物组,显示出改善的细菌形状和宿主微生物相互作用.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 牙周病学 牙周病学
背景情况:
- 初级免疫缺陷 (PID) 是一种影响免疫功能的罕见遗传疾病.
- PIDs可能会危及牙斑控制和口腔健康.
- 中性,一些PID的共同特征,影响免疫反应.
研究的目的:
- 在患有PID的儿童中,描述下牙牙斑微生物群.
- 将PID儿童的微生物组与健康对照组进行比较.
- 评估非手术性牙周治疗对PID微生物群的影响.
主要方法:
- 从PID儿童和健康对照者的牙和切口中收集了下牙斑块.
- 利用Illumina对16SrRNA基因 (V1-V2区域) 的metabarcoding进行微生物组概况.
- 在基线和治疗后6个月分析了微生物社区结构 (α和β多样性).
主要成果:
- 非手术性牙周治疗显著改变了PID儿童的下微生物社区结构.
- 增加了Rothia spp.,Neisseria spp. 和Actinomyces spp. 的丰富性. 与临床改善相关联.
- 在PID儿童中基线中性粒细胞计数显示与特定细菌属的关联,表明宿主-微生物关系.
结论:
- 非手术性牙周治疗有效调节了中性贫血PID儿童的口腔微生物组.
- 确定了与口腔微生物群相关的新型宿主-微生物相互作用,对健康和疾病的影响.
- 这些发现突显了牙周治疗在PID患者口腔健康管理中的潜力.
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