相关实验视频

Updated: Sep 14, 2025

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants

Published on: July 1, 2018

[为植物构建多基因表达系统并验证其功能]

Yin-Yin Jiang¹, Ya-Nan Tang¹, Yu-Ping Tan²

¹State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica

|July 21, 2025

概括

一种新的植物表达向量使得高效的多基因共同转化成为可能. 这种系统允许灵活的基因插入,并成功地产生了贝塔宁,为植物代谢工程和途径分析提供了可靠的平台.

科学领域:

植物生物技术植物生物技术
分子生物学分子生物学
基因工程是一种基因工程.

背景情况:

有效的多基因表达系统对于植物基因工程至关重要.
现有的系统往往缺乏灵活性和易于操作的多个基因插入.

研究的目的:

为植物设计和构建一种新,高效和用户友好的多基因表达系统.
用记者基因和目标化合物合成途径验证系统的功能.

主要方法:

植物表达载体 (pC1300-TP2-P19) 的构建,其中包含三个独立的基因表达录音带和基因沉默抑制剂 (P19).
灵活插入基因通过酶消化/结合或再组合,具有蛋白质标记的选项.
使用增强绿色光蛋白 (eGFP) 记者和在烟草中共表达贝塔宁合成途径基因 (BvCYP76AD,BvDODA1,DbDOPA5GT) 的验证.

主要成果:

从每张录音带使用eGFP在烟草短暂表达中证明了独立和正常的表达.
三种贝塔宁合成基因的成功联合表达和高水平.
通过表型分析,西部污染和化学检测确认了目标化合物贝塔宁的产生.

结论:

开发的多基因表达系统高效,可靠,并且能够共同转化多个植物基因.
为植物天然产品路径分析,功能基因验证和代谢工程提供了多功能矢量平台.
通过各种蛋白质标签促进了目标基因的灵活插入和检测.

关键词:

美国GFP的GFP是GFP.贝塔尼尼贝塔尼尼贝塔尼多基因表达的表达方式短暂的表达方式短暂的表达方式矢量构造构造是一种矢量构造.

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