结合性酸交叉链接和酸封顶用于减轻牛心周内的化
Dinesh Annamalai1,2, Thiyaneshwar Ramamoorthy1, Agnes Mary Soloman1
1Biochemistry and Biotechnology Laboratory, CSIR-Central Leather Research Institute (CSIR-CLRI), Sardar Patel Road, Adyar, Chennai, 600020, India.
开发持久的生物假体心脏门 (BHVs) 涉及去细胞化牛心膜 (BP) 并使用与谷氨酸封闭的glyoxal交联. 这种新的方法提高了BHV的抗化能力和生物相容性.
科学领域:
- 生物材料科学 生物材料科学
- 心血管工程 心血管工程
- 组织工程是组织工程.
背景情况:
- 生物假体心脏门 (BHVs) 由于退化和化而面临限制.
- 目前的材料需要提高长期耐用性和生物相容性.
研究的目的:
- 使用脱细胞牛心脏 (BP) 开发一种更耐用的BHV材料.
- 为了研究除了甲之外的其他交叉链接策略.
主要方法:
- 牛心膜 (BP) 通过使用脱氧化 (SDC) 和Triton X-100 (TX) 进行脱细胞化.
- 脱细胞化血压与谷氨基,谷氨酸,酸或 катехин交叉链接.
- 对于每个交叉连接方法,评估了化倾向和机械性能.
- 格里奥克萨尔交叉链接的BP进一步被限制在l-glutamic酸上.
主要成果:
- SDC-TX脱细胞化有效地去除了细胞成分,同时保留了细胞外基质 (ECM).
- 具有glyoxal交叉链接,具有l-glutamic acid封闭,显示出对化具有优越的抵抗力.
- 经过格里奥萨尔治疗的BP的机械性能仍然与经过甲治疗的门相似.
结论:
- 用SDC-TX脱细胞化BP与glyoxal交叉链接,并用l-glutamic acid封顶是一种有前途的策略.
- 这种方法为生物假体心脏门提供了增强的耐用性和生物相容性.
- 它为传统的基于谷氨的治疗提供了潜在的替代方案.
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