在肠道环境中,在合成益生菌中消除基因的遗传保护措施
Nhu Nguyen1, Miaomiao Wang1, Lin Li1
1Department of Biomedical Engineering, University of North Texas, Denton, TX, USA.
iScience
|July 21, 2025
概括
工程化益生菌可以安全地使用一种新型电路来控制,该电路会降解它们的遗传物质,而不是杀死细胞. 这种系统只在需要时确保益生菌的活性,防止不良影响.
科学领域:
- 合成生物学 合成生物学
- 微生物工程 微生物工程
- 基因电路的基因
背景情况:
- 工程微生物具有治疗潜力,但需要强大的安全机制.
- 现有的生物控制策略通常依赖于细胞杀死,这可能会影响性能.
- 来自生物封闭的细胞毒性可能会阻碍工程益生菌的适应性和有效性.
研究的目的:
- 开发和评估一种用于工程益生菌的新型生物控制系统.
- 设计一个电路,降解工程遗传物质,而不是杀死宿主细胞.
- 为了确保工程益生菌菌株的受控活性和安全停用.
主要方法:
- 设计了一个两层的转录调节电路.
- 整合了CRISPR系统,以准和降低特定的工程基因.
- 该系统是在Escherichia coli Nissle 1917 (EcN) 中实施的.
- 评估了生物控制的有效性和对细胞健康的影响.
主要成果:
- 生物封闭电路没有影响 *EcN* 益生菌的适应性.
- 该系统有效地降解了工程遗传材料和活动.
- 工程化益生菌在持续信号供应下维持了7天的功能.
- 在信号停止后的2天内,益生菌活性变得无法检测.
结论:
- 一个新的,非致命的生物控制系统已成功开发为工程益生菌.
- 这种基于CRISPR的电路提供了对工程遗传材料的精确控制.
- 该系统为安全有效地部署工程微生物提供了一个有希望的策略.
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