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准备,优化,和稳定性的异硫蓝载荷人血清白蛋白结构的纳米聚合物

Emre Ozgenc¹, Meliha Ekinci¹, Evren Gundogdu¹

¹Ege University, Faculty of Pharmacy Department of Radiopharmacy, Bornova, Izmir 35040, Turkey.

|July 21, 2025

概括

研究人员开发了带有异硫蓝 (ISB) 染料的稳定人血清白蛋白 (HSA) 纳米颗粒. 这些HSA纳米粒子提供了提高稳定性和药物输送的理想性质,克服了当前标记物的局限性.

科学领域:

生物材料科学生物材料科学
纳米技术纳米技术
制药科学制药科学

背景情况:

人类血清白蛋白 (HSA) 是适合于药物输送系统的蛋白质载体.
HSA纳米颗粒在各种疾病治疗中得到广泛使用.
对于HSA纳米粒子管理的关键参数包括粒子大小,表面电荷和药物负载.

研究的目的:

使用纳米沉方法开发基于HSA的纳米颗粒封装异硫蓝 (ISB) 染料.
为了优化配方参数,包括溶剂/非溶剂比率,pH值,交叉连接剂,染料和表面活性剂度.
评估开发的纳米粒子的物理化学特性和体外稳定性.

主要方法:

纳米沉程序用于HSA纳米粒子合成.
使用扫描电子显微镜 (SEM) 和动态光散射 (DLS) 进行表征.
优化配方参数和在3个月内进行体外稳定性评估.

主要成果:

从93.88到113.90纳米大小的球形纳米粒子已成功制备.
粒子大小分布 (PdI) 值为0.19和0.44,表面电荷为-26.0和57.4mV.
异硫蓝 (ISB) 的加载效率为15-20%,配方在3个月内表现出良好的稳定性.

结论:

开发的HSA纳米粒子提供了稳定的纳米合体结构,具有药物输送的理想特性.
这些纳米粒子克服了自由ISB的局限性,例如快速清除和光降解.
该研究成功地准备了ISB染料装载的HSA纳米粒子,适用于先进的治疗应用.

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