阻止CDC25B可以通过阻止细胞循环来抑制肉瘤的进展
Renqin Lin1,2,3, Jianhua Lin1,2,3
1Department of Orthopedics, The First Affiliated Hospital, Fujian Medical University, Fuzhou, China.
Translational cancer research
|July 21, 2025
概括
细胞分裂周期蛋白25B (CDC25B) 在软组织肉瘤中过度表达,与生存率较差相关. 抑制CDC25B通过诱导细胞循环停止来抑制肉瘤的进展,这表明它是潜在的治疗标.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 生物化学 生物化学
背景情况:
- 软组织肉瘤是一种罕见的恶性瘤,起源于介质细胞组织.
- 细胞分裂周期蛋白25B (CDC25B) 在肉瘤发病过程中的作用尚不清楚.
- CDC25B与各种人类疾病有关,突出显示其在癌症中的潜在相关性.
研究的目的:
- 为了研究CDC25B在肉瘤的预后意义.
- 探索瘤中准CDC25B的治疗潜力.
- 阐明CDC25B影响肉瘤进展的机制.
主要方法:
- 对CDC25B表达的生物信息分析及其与肉瘤生存结果的相关性.
- 在体外实验中使用CDC25B抑制剂 (CDC25B-IN-1) 评估其对肉瘤细胞的影响.
- 试验包括CCK-8,qPCR,EdU,殖民地形成,穿孔迁移,伤口愈合,流细胞计和西斑.
主要成果:
- 发现CDC25B在肉瘤组织中过度表达.
- 高的CDC25B表达与疾病特异性存活率,无进展间隔和整体存活率的下降显著相关.
- 抑制CDC25B抑制了肉瘤细胞的增殖和迁移,并诱导了G2细胞周期的停止,证实了其在进展中的作用.
结论:
- 疾病预防控制中心25B是一种针对肉瘤的独立预后生物标志物.
- 用抑制剂准CDC25B是一种有前途的治疗策略.
- CDC25B在细胞循环调节中的作用为其在肉瘤中的治疗向提供了机制基础.
相关概念视频
02:34Inhibition of Cdk Activity
4.9K
The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.9K
02:23Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
01:23Negative Regulator Molecules
36.0K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
36.0K
00:50Anaphase Promoting Complex
3.0K
The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...
3.0K
02:37DNA Damage can Stall the Cell Cycle
9.3K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.3K
01:20The Retinoblastoma Gene
4.2K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.2K