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相关概念视频

Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

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The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
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Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

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A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
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相关实验视频

Updated: Sep 14, 2025

In vivo Imaging of Biological Tissues with Combined Two-Photon Fluorescence and Stimulated Raman Scattering Microscopy
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表面增强拉曼光谱 (SERS) 基于生物和环境的2D和3D成像.

Qishen Huang1, Huiyuan Guo2, Wei Wang3

  • 1School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China.

ACS environmental Au
|July 21, 2025
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概括

表面增强拉曼光谱 (SERS) 成像为生物和环境样本提供敏感的实时分析. 应对其挑战将扩大SERS在诊断和污染物监测中的应用.

关键词:
这就是 SERS SERS.分析方法分析方法.生物感应生物感应环境传感 环境传感影像成像技术 影像成像技术微环境中的微环境.纳米探针是一种纳米探针.纳米结构是一种纳米结构.

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科学领域:

  • 分析化学 分析化学
  • 频谱学是一种光谱学.
  • 生物技术是生物技术.

背景情况:

  • 表面增强拉曼光谱 (SERS) 成像是一种强大的分子分析技术.
  • 它提供高灵敏度和空间分辨率用于in situ检测.
  • 在生物和环境研究中,SERS成像非常有价值.

研究的目的:

  • 审查SERS成像在生物和环境领域的应用.
  • 为了突出SERS技术在各种分析的进步.
  • 讨论SERS成像的挑战和未来潜力.

主要方法:

  • SERS成像利用等离子纳米结构来增强拉曼信号.
  • 技术包括纳米组装,生物对角化学和抗体结合.
  • 在体内研究中使用与其他成像方法的整合.

主要成果:

  • 在生物系统中,SERS成像成功检测核酸,蛋白质,生物标志物和病原体.
  • 环境应用包括追踪农药,纳米粒子和重金属.
  • 基于SERS的成像提供了pH和活性氧物种 (ROS) 的空间数据.

结论:

  • SERS成像提供了分析物的敏感检测和分布分析.
  • 为了更广泛的实施,需要解决当前的挑战.
  • 在污染物监测,临床诊断和生物分析方面,SERS成像具有显著的前景.