相关实验视频

Updated: Sep 14, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

使用16S和18SrRNA基因下一代测序对人类肠道微生物群的结构分析与单细胞肠道原生体相关

Salem Belkessa¹, Edoardo Pasolli², Bachir Medrouh³

¹Laboratory of Exploration and Valorization of Steppic Ecosystems, Department of Biology, Faculty of Nature and Life Sciences, Ziane Achour University of Djelfa, Moudjbara Road, BP 3117, Djelfa 17000, Algeria.

Computational and structural biotechnology journal

|July 21, 2025

概括

吉亚迪亚感染并没有改变肠道微生物群的多样性. 然而,细胞囊的共感染显著影响了微生物群落,这表明细胞囊可能会推动肠道微生物群的多样性.

科学领域:

微生物学微生物学
人类肠道微生物组研究研究
寄生虫学的寄生虫学

背景情况:

肠道微生物群在宿主健康和疾病中起着至关重要的作用.
虽然一些单细胞真核生物,如细胞囊,与肠道活化有关,但致病原生动物,如Giardia对微生物群的影响尚不清楚.
了解这些相互作用对于破译肠道健康至关重要.

研究的目的:

为了调查和比较肠道微生物群的个人Giardia阳性和Giardia阴性.
评估与其他真核生物 (Archamoebae,Blastocystis) 共同殖民对这些形状的影响.
为了确定Giardia或Blastocystis是否显著改变肠道微生物多样性.

主要方法:

利用基于片的新一代核糖体和真核糖体核糖体基因的测序.
分析了60名吉亚迪亚阳性和31名吉亚迪亚阴性阿尔及利亚人的肠道微生物群组成.
基于与Archamoebae和Blastocystis共同殖民的分层分析.

主要成果:

在Giardia阳性和阴性个体之间,无论Archamoebae或Entamoeba是否存在,都没有发现alpha和beta微生物群多样性的显著差异.
在Giardia阳性/母细胞瘤阴性和Giardia阳性/母细胞瘤阳性个体之间观察到α和β多样性的显著差异 (p=0.0016).
无论Giardia状态 (p<0.05) 是否存在,在阴性和阳性母细胞囊携带者之间也发现了类似的多样性差异.

结论:

仅靠giardia殖民似乎并没有显著改变肠道微生物群的多样性.
胚胎囊共同殖民与肠道微生物群多样性的显著变化有关,独立于Giardia状态.
胚囊细胞可能是肠道微生物群多样性的关键驱动因素,需要进一步研究.

关键词:

阿姆普利康 (Amplicon) 是一个交叉体是一个共体体.宿主-病原体相互作用微生物组是一个微生物组.原生动物原生动物单细胞原生体是一种单细胞原生体.

更多相关视频

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Guided Protocol for Fecal Microbial Characterization by 16S rRNA-Amplicon Sequencing

Published on: March 19, 2018

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Exploring the Root Microbiome: Extracting Bacterial Community Data from the Soil, Rhizosphere, and Root Endosphere

Published on: May 2, 2018

相关概念视频

Diversity of Protists I

Diversity of Protists I

Excavata is a diverse group of protists that includes both chemoorganotrophic and phototrophic species, with some thriving in anaerobic environments. Among the key groups within Excavata are diplomonads and parabasalids, which are flagellated protists that lack mitochondria and chloroplasts. These microorganisms typically inhabit anoxic environments, such as the intestines of animals, where they exist either symbiotically or as parasites, relying on fermentation for energy production. Some...

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...