口服微生物群调节和向炎症的聚合体凝用于性结肠炎的RNAi治疗
Huan He1, Xinyi Dong2, Li Cao1
1College of Pharmaceutical Sciences and State Key Laboratory of Radiation Medicine and Protection, Soochow University, Suzhou, China.
Bioactive materials
|July 21, 2025
概括
新的聚合体凝为性结肠炎提供RNA干扰疗法,调节肠道微生物群并减少炎症. 这种口服治疗在结肠炎模型中有效地恢复了肠道免疫和微生物平衡.
科学领域:
- 生物材料科学 生物材料科学
- 胃肠病学 胃肠病学
- 在RNA疗法方面.
背景情况:
- 性结肠炎 (UC) 治疗是具有挑战性的,因为复杂的病原和恶劣的胃肠环境.
- 目前的治疗方法在有效向炎症组织和调节肠道微生物群方面存在局限性.
研究的目的:
- 开发用于性结肠炎的向RNA干扰 (RNAi) 治疗的新型口服聚合物凝.
- 研究这些水凝在调节肠道微生物群和抑制肠道炎症方面的疗效.
主要方法:
- 开发自愈和可食用水凝 (SHE-Gel) 来自硫化藻酸盐和多巴胺修饰氧化胰岛素 (DA-OIn).
- 将酸和瘤亡因子α-特定siRNA封装聚合体 (GA-siTNFα-PS) 纳入SHE-Gel.
- 评估SHE-Gel的稳定性,降解以及DA-OIn的抗氧化和益生菌活性.
- 在大肠炎小鼠模型中评估GA-siTNFα-PS向传递,siTNFα释放和治疗效果.
主要成果:
- SHE-Gel在胃中表现出稳定性,在结肠中有针对性的降解,而DA-OIn表现出益生菌作用.
- GA-siTNFα-PS有效地将siTNFα传递给炎症结肠组织中的巨细胞,酸提供抗氧化保护.
- 在性结肠炎模型中,开发的聚合体水凝显著抑制了炎症,并保持了肠道微生物群的恒常性.
- 与抗TNFα抗体的联合治疗进一步恢复了晚期结肠炎模型中的肠道免疫和肠道微生物群平衡.
结论:
- 口服聚合体水凝通过结合微生物群调节和向性炎症抑制,为性结肠炎治疗提供了一种多功能策略.
- 这种方法有效地阻止了炎症的进展,并恢复了肠道平衡,为炎症性肠道疾病提供了一个有前途的治疗途径.
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