在唾液样本中净化和缩Mycobacterium tuberculosis的一种方法:双膜过和缩方法 (DMFCM)
Dingyi Liu1, Wenjing Wang1, Yue Hou1
1Translational Medicine Center, Beijing Chest Hospital, Capital Medical University, Beijing 101149, China.
概括
一种新的双膜过度度方法 (DMFCM) 有效地净化和缩结核菌 (M.tb) 在唾液中. 这提高了诊断灵敏度,减少了结核病 (TB) 检测的时间.
科学领域:
- 微生物学 微生物学
- 传染性疾病 传染性疾病
- 诊断科学 诊断科学 诊断科学
背景情况:
- 结核病 (TB) 是一个重要的全球公共卫生威胁.
- 在临床样本中检测Mycobacterium结核病 (M.tb) 是具有挑战性的,因为细菌负载和度较低.
- 这种困难影响了实验室结核病诊断的灵敏度和准确性.
研究的目的:
- 评估双膜过度度方法 (DMFCM) 在临床唾液样本中净化和缩M.tb的有效性.
- 将DMFCM的性能与现有的结核病检测方法进行比较.
主要方法:
- 采用双膜过度度方法 (DMFCM) 来处理临床样.
- 使用显微镜,定量实时PCR (qPCR) 和液态培养 (MGIT 960) 分析了纯化和缩的M.tb样本.
- 结果与直接涂抹显微镜和标准离心法进行了比较.
主要成果:
- DMFCM从80.53%的标本中去除了40-80%的杂质,使M.tb的度高达3.366倍.
- 显微镜敏感度从58.33% (直接涂抹) 增加到73.61%使用DMFCM.
- 用DMFCM处理的标本在qPCR (37/42比35/42) 中显示出更高的阳性率,在MGIT 960培养中显示出更快,更积极的结果.
结论:
- 在唾液样本中,DMFCM有效地净化和缩M.tb.
- 这种增强的纯度和度显著提高了结核病诊断的阳性检测率.
- DMFCM提供了一种有前途的方法来提高实验室结核病检测的灵敏度和效率.
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