东方猫跳的分子特征,Ctenocephalides orientis,基于核和线粒体蛋白质编码基因寄生于山羊
Muhammad Shair Zaman Ghauri1,2, Samiullah Soomro1,2, Apinya Arnuphapprasert3
1Center of Excellence in Veterinary Parasitology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Current research in parasitology & vector-borne diseases
|July 21, 2025
概括
在泰国发现东方猫 (Ctenocephalides orientis) 感染了山羊,扩大了它们已知的宿主范围,超出了伴侣动物范围. 这一发现突出了它们在畜牧群体疾病传播中的潜在作用.
科学领域:
- 寄生虫学和媒介性疾病
- 分子遗传学和基因组学
- 兽医昆虫学 兽医昆虫学
背景情况:
- 跳是影响人类和动物的疾病的显著外寄生虫载体.
- 东方猫跳 (Ctenocephalides orientis) 在亚洲很常见,主要感染狗.
- 它的存在和对小反动物 (如山羊) 的影响尚不清楚.
研究的目的:
- 调查泰国感染山羊的跳的流行率和遗传特征.
- 用形态和分子方法确认跳的物种识别.
- 通过使用核和线粒体标记来分析Ctenocephalides orientis的遗传关系.
主要方法:
- 从2017年1月到2023年10月,在泰国七个省份的山羊养殖场进行了跳调查.
- 使用形态密钥和分子分析识别的跳标本,分析了细胞染色体c氧化酶子单元1 (cox1),细胞染色体c氧化酶子单元2 (cox2),内部转录间隔器1 (ITS1) 和延长因子-1α (EF-1α) 基因.
- 测序和分析了Ctenocephalides orientis的部分线粒体基因组,并进行了植物遗传学分析.
主要成果:
- 在500只被检查的山羊中,33只 (6.6%) 感染了跳,这些跳只被确定为Ctenocephalides orientis.
- 分子标记物,特别是EF-1α,揭示了C. orientis种群中的遗传多样性.
- 遗传学分析证实了C. orientis的独特进化位置和与Ctenocephalides canis.的密切关系.
结论:
- 在泰国,Ctenocephalides orientis感染了山羊,扩大了其已知的宿主范围,超出了伴侣动物.
- 这项研究为未来的跳研究提供了有价值的分子数据 (核和线粒体标记).
- 这些发现强调了C. orientis作为牲畜和其他动物疾病的载体的潜力.
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