Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

The Tumor Microenvironment02:17

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
Metastasis02:30

Metastasis

Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Cancer Cell Migration through Invadopodia01:35

Cancer Cell Migration through Invadopodia

Invadosome is a broad category of cell surface structures with proteolytic activity that  degrades the extracellular matrix (ECM). Invadosomes are present in normal cell types, including macrophages, endothelial cells, and neurons, as well as tumor cells. Although the macrophage podosomes and tumor cell invadopodia are classified as invadosomes, they have different structures, molecular pathways, and functions. Podosomes are short structures that last for a few minutes. However, invadopodia can...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Near-Real-Time Clinical Trial Accrual Dashboard in a National Cancer Institute-Designated Cancer Center: Mixed Methods Implementation Study.

JMIR medical informatics·2026
Same author

Characterization of Isoorientin and Paeoniflorin as Botanical Glucocorticoid Receptor Modulators from White Peony and Chasteberry.

Nutrients·2026
Same author

The Human Gut Microbiome Metabolizes Diverse Bioactive Coumarins via α,β-Unsaturated Lactone Reduction.

Journal of natural products·2026
Same author

Ciliated Cells Drive Critical STING-Mediated Tumor Suppression in the Fallopian Tube Epithelium.

Cancer research·2026
Same author

Comparative single-cell atlases reveal injury-driven tubal epithelial regeneration as a window for ovarian carcinoma initiation.

bioRxiv : the preprint server for biology·2026
Same author

Activity of Didesmethylrocaglamide in High Grade Serous Ovarian Cancer Using Preclinical In Vitro and In Vivo Models.

Journal of natural products·2026

相关实验视频

Updated: Jul 5, 2026

Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells
11:46

Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells

Published on: May 9, 2011

19.8K

通过模拟卵巢癌癌前癌情景,定义由细胞外膀驱动的输卵管上皮质重编程.

Jared Sipes1,2, Didi Zha3, Sagar Rayamajhi1

  • 1Department of Pathology and Laboratory Medicine, University of Kansas Medical Center, Kansas City, Kansas.

Cancer research communications
|July 21, 2025
PubMed
概括

卵巢癌小细胞外囊泡 (sEVs) 改变人类输卵管上皮质 (hFTE) 基因表达,促进免疫信号变化. 这种微流体模型揭示了卵巢管中早期病原发生的事件,卵巢癌的起源.

更多相关视频

Heterotypic Three-dimensional In Vitro Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression
12:42

Heterotypic Three-dimensional In Vitro Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression

Published on: August 28, 2012

15.2K
Author Spotlight: Advancing Human Ovarian Repair and Cancer Studies with Surface Epithelium Organoids
07:37

Author Spotlight: Advancing Human Ovarian Repair and Cancer Studies with Surface Epithelium Organoids

Published on: August 16, 2024

1.5K

相关实验视频

Last Updated: Jul 5, 2026

Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells
11:46

Ex Vivo Culture of Primary Human Fallopian Tube Epithelial Cells

Published on: May 9, 2011

19.8K
Heterotypic Three-dimensional In Vitro Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression
12:42

Heterotypic Three-dimensional In Vitro Modeling of Stromal-Epithelial Interactions During Ovarian Cancer Initiation and Progression

Published on: August 28, 2012

15.2K
Author Spotlight: Advancing Human Ovarian Repair and Cancer Studies with Surface Epithelium Organoids
07:37

Author Spotlight: Advancing Human Ovarian Repair and Cancer Studies with Surface Epithelium Organoids

Published on: August 16, 2024

1.5K

科学领域:

  • 生殖生物学 生殖生物学
  • 癌症研究 癌症研究
  • 生物技术是生物技术.

背景情况:

  • 在人类输卵管表皮 (hFTE) 中的血清管内皮癌 (STIC) 是高度血清卵巢癌的前体.
  • 小型细胞外囊泡 (sEVs) 在正常和癌症组织中介导关键信号传递.
  • 研究sEV对hFTE影响的ex vivo系统是有限的.

研究的目的:

  • 开发和利用一个微流体平台来研究sEV-hFTE相互作用.
  • 分析hFTE对卵巢癌sEVs的短期转录组和长期蛋白组反应.
  • 为了研究卵巢癌发病的早期分子变化在输卵管内.

主要方法:

  • 一个微流体组织培养平台是为hFTE设计的.
  • 组合的空间转录组学和蛋白质组学被用于双响应分析.
  • 卵巢癌衍生的sEVs被用于刺激hFTE培养1和14天.

主要成果:

  • 短期 (1天) sEV暴露改变了hFTE分泌细胞中的68个转录,包括与免疫相关的基因 (例如CXCL,VCAM1,NFKB1,IL1B).
  • 长期 (14天) 的sEV暴露改变了hFTE的二次释放EV中的7个转录和25个EV货物蛋白.
  • 卵巢癌sEVs被证明可以重新连接目标细胞信号,并修改输卵管免疫格局.

结论:

  • 卵巢癌衍生的sEV诱导hFTE显著的转录组和蛋白组变化.
  • 微流体平台有效地模拟了卵巢癌早期在输卵管中的病原发生.
  • 这些发现提供了对EV驱动的细胞信号重编程的见解,hFTE是卵巢癌的起源组织.