变压器在Bemisia tabaci中参与了女性的性别决定
Yating Liu1, Wenlu Wang2, Yina Wang1
1School of Agricultural and Biological Engineering, Foshan University, Foshan, People's Republic of China.
Insect molecular biology
|July 21, 2025
概括
变压器 (tra) 基因对于甜白的雌性发育至关重要. Bttra沉默影响双性 (dsx) 表达,揭示了它在这种侵入性害虫中的作用.
科学领域:
- 发育生物学是发展生物学.
- 进化生物学是进化的生物学.
- 昆虫分子生物学 昆虫分子生物学
背景情况:
- 昆虫的性别决定机制是多样化的,但变压器 (tra) +变压器-2 (tra2) →双性 (dsx) 途径是保留的.
- 在侵入性害虫Bemisia tabaci中,tra2和双性 (dsx) 在男性生殖器形成中的作用已知,但变压器 (tra) 的功能仍然不清楚.
研究的目的:
- 为了表征Bemisia tabaci.中的变压器 (Bttra) 基因.
- 调查Bttra和Bttra之间的相互作用2.
- 阐明Bttra在B. tabaci.的性别决定和女性发育中的作用.
主要方法:
- 描述Bttra,包括确定TRA/TRA-2结合点和拼接变体.
- 在体外谷氨S转移酶 (GST) 下拉试验以确认BtTRA和BtTRA2的相互作用.
- 通过Bttra基因沉默进行功能分析,以评估其对Btdsx表达和女性发育的影响.
主要成果:
- 对Bttra.多个TRA/TRA-2结合点和几个拼接变体被确定为Bttra.
- 在体外证实了BtTRA和BtTRA2之间的直接相互作用.
- Bttra沉默证明了对Btdsx表达的调节作用,对B. tabaci. 的雌性发育至关重要.
结论:
- 变压器 (tra) 基因在Bemisia tabaci的雌性发育中起着至关重要的作用.
- 这项研究突出了平分类昆虫中性别确定途径的差异.
- 这些发现为管理Bemisia tabaci种群提供了潜在的分子标.
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