细胞粘附分子 Echinoid 促进组织存活,并单独限制组织过度生长
Danielle C Spitzer1, William Y Sun1, Anthony Rodríguez-Vargas1
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA 94720-3200, USA.
概括
德洛索菲拉细胞中状 (ED) 功能减少会导致细胞亡和消除,但也会因无法阻止大小而过度生长. (ED) 蛋白对于细胞存活和适当的生长停止至关重要.
科学领域:
- 发展生物学 发展生物学
- 细胞生物学 细胞生物学
- 遗传学 遗传学 是一个
背景情况:
- 细胞生长和存活是由内在特性和细胞间相互作用调节的.
- 脊 (ED) 基因编码了一种细胞表面蛋白质,参与调节这些过程.
研究的目的:
- 为了研究Drosophila影像盘中的状骨 (ED) 的功能.
- 阐明功能减弱对细胞存活,细胞亡和生长的影响的机制.
主要方法:
- 使用RNA干扰 (RNAi) 在马赛克Drosophila影像盘中进行基因选,以击败编码细胞表面蛋白的基因.
- 分析了 echinoid (ed) knockdown 对克隆大小,形状,存活率和亡的影响.
- 在ED突变细胞和野生类型细胞中评估Hippo通路活性和Diap1蛋白水平.
主要成果:
- 减少状 (ED) 功能导致更小,更少的克隆,这些克隆经常由于增加的亡而被消除,与减少的Diap1蛋白有关.
- 在ED突变细胞的野生类型邻居中,河马通路活性下降,这表明细胞竞争.
- 主要具有突变组织的磁盘由于增长停止失败而表现出过度生长,尽管亡增加了.
结论:
- 虫有两种不同的功能:维持Diap1水平以产生抗亡作用,并调节适当的最终尺寸时的生长停止.
- 细胞竞争机制有助于消除突变克隆.
- 虫 (ED) 在发育过程中在平衡细胞存活和生长控制方面发挥着关键作用.
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