在金属有机框架上调整微环境,以实现高效的酶固定和敏感的免疫测试
Guo Chen1,2, Weiqing Xu1, Wenling Gu1
1State Key Laboratory of Green Pesticide, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, P. R. China.
Analytical chemistry
|July 21, 2025
概括
我们开发了功能化的层次性多孔金属有机框架 (HP-MOFs) 用于酶固定. 这种方法提高了酶的稳定性和活性,从而产生了一种敏感的生物传感器,用于在低水平上检测pyrifos.
科学领域:
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
- 纳米技术 纳米技术
背景情况:
- 在金属有机框架 (MOF) 中的酶固定对于在恶劣条件下的酶稳定性至关重要.
- 然而,MOF中低于最佳的微环境往往会降低酶的生物活性和实际效用.
研究的目的:
- 开发一种功能化的层次性多孔MOF (HP-MOF) 载体,以实现高效的酶固定.
- 增强MOF内的微环境,以提高酶的性能和稳定性.
- 使用固定酶创建一个敏感的生物传感器,用于pyrifos检测.
主要方法:
- 功能化的连接物被引入到层次性多孔MOF (HP-MOF) 中,以调整微环境.
- 用一种酸蚀刻策略来进一步增强中孔结构.
- 细胞染色体c (Cyt c) 被固定在功能化的HP-MOF上 (HP-MOF-OH@Cyt c).
- 使用HP-MOF-OH@Cytc构建了一个生物传感器,用于检测pyrifos.
主要成果:
- 惠普-MOFs的孔径与细胞染色体c相匹配,允许高负载 (19.00%) 和酶可访问性.
- 水友性-OH功能化保留了Cytc的二次结构,增强了催化活性.
- 固定化的酶显示出出色的可回收性和对极端条件的耐受性.
- 由此产生的生物传感器显示了pyrifos检测的广泛线性范围 (1010,000 pg mL−1) 与低检测极限 (4.63 pg mL−1).
结论:
- 功能化的HP-MOF为酶固定提供了优化的微环境,增强了稳定性和活性.
- 开发的HP-MOF-OH@Cytc系统有效地创建了强大而敏感的生物传感器.
- 这种方法对基于酶的实际应用有很大的前景,特别是在环境监测中.
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