通过两种益生菌Lactobacillus菌株对抗 Clostridioides difficile 的抗生素殖民后耐药性的微分调节
Matthew H Foley1,2, Arthur S McMillan1, Sarah O'Flaherty2
1Department of Pathobiology and Population Health, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina, USA.
mBio
|July 21, 2025
概括
某些益生菌可能会损害抗生素后的肠道恢复. 乳酸菌促进了对Clostridioides difficile的殖民抵抗力,与L. acidophilus不同,通过丰富有益的Muribaculaceae细菌.
科学领域:
- 微生物学 微生物学
- 胃肠病学 胃肠病学
- 免疫学 免疫学 免疫学
背景情况:
- 益生菌通常用于抗生素后预防腹疾病,通常是由Clostridioides difficile引起的.
- 益生菌对抗生素治疗后肠道微生物群的恢复的影响尚不清楚.
- 之前的研究经常将益生菌的益处概括,而不考虑菌株对病原体耐药性的特定影响.
研究的目的:
- 在接受抗生素治疗的小鼠模型中,研究Lactobacillus acidophilus和Lactobacillus gasseri对抗 Clostridioides difficile的殖民抗性的影响.
- 为了确定特定的益生菌菌株是否影响抗生素暴露后肠道微生物群的恢复.
- 鉴定肠道细菌与增强的殖民抵抗后益生菌的管理.
主要方法:
- 接受抗生素治疗的小鼠接受了单次剂量的Lactobacillus acidophilus NCFM或Lactobacillus gasseri Lg-36.
- 评估了对Clostridioides difficile感染的殖民耐药性.
- 用测序技术分析了肠道微生物群的组成.
- 进行了体外实验,以评估特定细菌对Clostridioides difficile生长的抑制作用.
主要成果:
- 乳杆菌酸性的管理恶化了Clostridioides difficile感染和受损的殖民抵抗恢复.
- 乳酸菌减少了Clostridioides difficile的殖民化,并促进了殖民抗性,可能是通过生产细菌素.
- 在L. gasseri给药后,观察到Muribaculaceae家族的丰富,包括Muribaculum intestinale和Duncaniella muris,并与改善的殖民抵抗有关.
- 这些Muribaculaceae物种在实验室中显示抑制了Clostridioides difficile的生长.
结论:
- 特定的益生菌菌株可以对肠道微生物群的恢复和抗生素后的病原体耐药性产生差异性,有时甚至是有害的影响.
- 乳酸菌 (Lactobacillus gasseri) 在恢复殖民耐药性方面表现有前途,可能是通过细菌素活性和促进有益的Muribaculaceae的生长.
- 未经充分研究的Muribaculaceae家族可能在预防Clostridioides difficile感染方面发挥重要作用.
- 需要进一步的研究来了解复杂的微生物社区相互作用以及益生菌在抗生素中断后影响肠道健康的精确机制.
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