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绝杀糖代谢途径使得高效的mycolicibacterial植物转换使用糖作为cosovlent

Shikui Song¹, Ye Wang^2,3, Jinpeng Yu³

¹Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, College of Life Sciences, Xinjiang Normal University, Urumqi, 830054, Xinjiang, China.

Applied microbiology and biotechnology

|July 21, 2025

概括

通过淘汰Mycolicibacterium neoaurum中的糖代谢途径,可以使用糖和HP-β-CD进行高效的植物转化. 这种策略可以增强类固醇中间体的产生,而不会影响细菌的生长.

科学领域:

生物技术是生物技术.
微生物工程微生物工程
类固醇的生物转化

背景情况:

菌根菌的植物转化对于生产类固醇中间体至关重要.
目前使用植物油或基基β-CD (HP-β-CD) 的方法需要高度,影响效率和回收.
在优化发酵介质以实现高效的植物生物转化方面存在挑战.

研究的目的:

开发一种新的策略,在Mycolicibacterium neoaurum.中有效转化植物.
调查糖醇代谢途径在植物转化中的作用.
优化发酵条件,以提高类固醇中间体的生产.

主要方法:

生物信息分析以确定糖代谢途径 (GLPK和ADHC).
基因淘汰实验创建一个双重突变 (HGMS6^{Δadhc/Δglpk}) 缺乏糖代谢.
在水相发酵中对突变植物的转化效率的评估,使用不同的甘油和HP-β-CD度.
试点规模的发酵试验,以评估转换率.

主要成果:

一个双重淘汰突变HGMS6^{Δadhc/Δglpk}成功地被创造出来,缺乏甘油代谢,但保持了生长.
在发酵介质中使用10%的甘油和0.5%的HP-β-CD实现了最佳的转化效率.
试验规模的发酵表明,植物醇的高转化率高达83.8% (mol/mol%).
突变者的表现与使用植物油或HP-β-CD的传统方法相匹配.

结论:

禁用糖代谢途径是一种可行的策略,可以增强菌根菌的植物转化.
甘油可以作为一种有效的共同溶剂在发酵介质中,用于制造类固醇中间体.
这种方法为提高类固醇生物转化过程的效率和可持续性提供了一个有希望的途径.

关键词:

这种细菌是Mycolicibacterium sp. 压力压力压力压力葡萄糖的代谢葡萄糖的代谢糖脱酶是一种糖脱酶.甘油酶酶是一种甘油激酶.植物醇转化转化

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