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Updated: May 8, 2026

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
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3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

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计算方法来划定溶酶体和相关的细胞内流通囊泡在对焦和其他光数据集中的计算方法

Charles Ellis1, David S Chatelet2, J Arjuna Ratnayaka3

  • 1Clinical and Experimental Sciences, Faculty of Medicine, University of Southampton, MP806, Southampton, UK.

Methods in molecular biology (Clifton, N.J.)
|July 21, 2025
PubMed
概括

分析细胞内贩运图像需要仔细选择方法. 这项研究比较了斐济,CellProfiler和Icy的溶酶体分析,强调结果不可互换.

关键词:
在3D成像中使用3D成像.计算 计算 计算 计算同焦点显微镜的共聚焦显微镜数据完整性数据完整性lysosome 是一个溶解体.视网膜色素上皮质 (RPE) 是一种干细胞是一种干细胞.贩卖黄囊细胞的贩运行为时间 量 量 量 量

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相关概念视频

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...