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Updated: Sep 14, 2025

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
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由单个突变引起的形状变化驱动的新德里金属β-乳糖酶的抑制剂依赖性耐受性

Liwen Huang1,2,3,4, Tsz-Fung Wong1,2, Qipeng Cheng2,5

  • 1Department of Applied Biology and Chemical Technology, Food Safety and Technology Research Centre, and Research Centre for Chinese Medicine Innovation, The Hong Kong Polytechnic University, Hung Hom, Kowloon 999077, Hong Kong SAR, China.

Journal of the American Chemical Society
|July 21, 2025
PubMed
概括

新德里金属β-乳糖酶 (NDM) 变种通过构造变化进化,M154L突变通过调节蛋白质动态来增强耐药性. 这揭示了设计NDM抑制剂的新策略.

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