揭示了早期玉米内和种子外分化的染色质可访问性动态
Mengyao Li1, Xiaoxing Fan2, Xiaohan Li1
1State Key Laboratory of Wheat Improvement, College of Life Sciences, Shandong Agricultural University, Tai'an, 271018, China.
The Plant journal : for cell and molecular biology
|July 21, 2025
概括
这项研究绘制了玉米核的发展图,揭示了染色质可访问性变化如何影响基因调节的产量和质量. 确定了影响粉和粉合成的新型因素.
科学领域:
- 植物生物学 植物生物学
- 基因组学就是基因组学.
- 发展生物学 发展生物学
背景情况:
- 玉米核的开发是产量和谷物质量的关键.
- 了解组织特异性调节网络需要破译染色质可访问性动态.
研究的目的:
- 通过使用集成的多组学来探索早期玉米核发育期间的染色质可访问性动态.
- 确定控制细胞命运转变和基因表达的调控机制.
主要方法:
- 对转化酶可访问的染色体测序 (ATAC-seq) 和RNA测序 (RNA-seq) 的测试在受精后4-10天 (DAP) 的内精和种子外衣样本上进行.
- 综合的多组学分析被用来绘制染色质可访问性和基因表达特征.
主要成果:
- 在早期内分化 (4-10 DAP) 期间,染色质可访问性经历了阶段特定的重塑.
- 鉴定了和粉合成途径的独特调节机制,与典型的共同调节不同.
- 新的转录因子 (例如DOF36,ZmNAC128,ZmbZIP3) 和下游基因被确定并验证.
- 在孕产妇的胎盘 chalaza (PC) 融合区和基底内精转移层 (BETL) 区域之间观察到类似的可访问色素区域 (ACR) 和表达模式,表明共同调节模块.
结论:
- 为了玉米核的开发,生成了ACR和转录组的时间进程地图.
- 该图谱有助于分析特定的基因表达调节,构建细胞分化轨迹,并开发细胞类型特定的基因调节网络.
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