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从酵母Ogataea polymorpha中在45°C的氧化糖中选择有效的乙醇生产者

Roksolana Vasylyshyn^1,2, Justyna Ruchala¹, Kostyantyn Dmytruk²

¹Faculty of Biotechnology, Medical College, University of Rzeszow, 35-601, Rzeszów, Poland.

Scientific reports

|July 21, 2025

概括

研究人员通过使用酵母Ogataea polymorpha.使用西洛斯和L-阿拉比诺斯来增强乙醇生产. 基因改造导致乙醇产量增加了50倍,显示出工业应用的潜力.

科学领域:

生物技术是生物技术.
微生物发酵微生物发酵
合成生物学合成生物学

背景情况:

奥加泰亚多态菌 (Ogataea polymorpha) 是一种耐热酵母,能够利用西洛斯.
为了节省成本,有效地生产生物燃料,有效发酵西洛斯和L-阿拉比诺斯至关重要.
目前用于改善O. polymorpha中酸发酵的方法需要优化.

研究的目的:

开发一种用于隔离O. polymorpha突变的阳性选择方法,使用增强的西洛斯和L-阿拉比诺斯发酵.
为了确定负责提高发酵能力的遗传修饰.
评估工程菌株对工业规模乙醇生产的潜力.

主要方法:

作为唯一的碳来源,对L-阿拉比诺酸进行突变的积极选择.
高性能突变菌株的全基因组测序.
对已识别的基因进行基因操纵 (基因破坏和过度表达).
在高温 (45°C) 进行发酵试验.

主要成果:

鉴定出一种突变菌株,A107,具有显著改善的西洛斯发酵.
全基因组测序揭示了API1和IRA1基因中的突变.
破坏IRA1增加了从两种糖的乙醇生产.
过度表达API1特别增强了L-阿拉比诺酸发酵.
最具生产力的突变物获得了20.91g/L的乙醇,比野生类型增加了50倍.

结论:

一种新的选择方法有效地识别了优质的西洛斯和L-阿拉比诺斯发酵突变物.
针对API1和IRA1的基因修改是提高O. polymorpha.pentose发酵的关键.
工程化O.多态菌株显示出工业乙醇生产的巨大潜力,特别是在同时糖化和发酵 (SSF) 过程中.

关键词:

在API1基因中,基因是API1.在IRA1基因上.酒精发酵的酒精发酵方式对于L-阿拉比诺糖来说,反向遗传学是一种反向的遗传学.

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