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相关概念视频

Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

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Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
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Translational Regulation01:29

Translational Regulation

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Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
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Ribosome Profiling02:24

Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Riboswitches01:56

Riboswitches

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Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
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Types of RNA01:23

Types of RNA

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Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
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Directing Proteins to the Rough Endoplasmic Reticulum01:34

Directing Proteins to the Rough Endoplasmic Reticulum

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The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
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相关实验视频

Updated: Sep 14, 2025

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
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Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

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CacyBP/SIP - RPL6相互作用:对核糖体功能的潜在影响

Ewelina Jurewicz1, Małgorzata Maksymowicz-Trivedi1, Omid Saberi-Khomami1

  • 1Nencki Institute of Experimental Biology, Polish Academy of Sciences, 3 Pasteur Street, Warsaw, 02-093, Poland.

Amino acids
|July 21, 2025
PubMed
概括
此摘要是机器生成的。

CacyBP/SIP与核糖体蛋白RPL6直接相互作用,影响核糖体功能和蛋白质合成. 沉默CacyBP/SIP降低了新生的多合成,并改变了神经母细胞瘤细胞的应激反应.

关键词:
CacyBP/SIP 这种植物.分子建模分子建模蛋白质合成 蛋白质合成在RPL6中,我们可以选择RPL6.核糖体 核糖体 核糖体 核糖体

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Eukaryotic Polyribosome Profile Analysis
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Eukaryotic Polyribosome Profile Analysis

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RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
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RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing

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相关实验视频

Last Updated: Sep 14, 2025

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling
06:58

Global Identification of Co-Translational Interaction Networks by Selective Ribosome Profiling

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Eukaryotic Polyribosome Profile Analysis
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Eukaryotic Polyribosome Profile Analysis

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RIBO-seq in Bacteria: a Sample Collection and Library Preparation Protocol for NGS Sequencing
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科学领域:

  • 分子生物学分子生物学
  • 细胞生物学 细胞生物学
  • 生物化学 生物化学

背景情况:

  • CacyBP/SIP (也称为CacyBP) 与NPM1有先前确定的相互作用,NPM1是核糖体生物发生的关键参与者.
  • 这项研究研究了CacyBP/SIP在更广泛的核糖体生物发生和功能中的作用.

研究的目的:

  • 确定CacyBP/SIP对核糖体生物发生和功能的影响.
  • 为了确定CacyBP/SIP.的潜在核糖体蛋白标.
  • 阐明CacyBP/SIP-核糖体相互作用对蛋白质合成的功能后果.

主要方法:

  • 质谱测量用于识别CacyBP/SIP交互的合作伙伴.
  • 生物化学测定证实了CacyBP/SIP和RPL6.6之间的直接相互作用.
  • 在分析以映射蛋白质结合域.
  • 标记O-propargyl-puromycin (OPP) 标签以评估新生的多合成.
  • 热冲击实验用于评估CacyBP/SIP沉默细胞中压力诱导的蛋白质生产 (Hsp70).

主要成果:

  • 质谱学确定了几种核糖体蛋白 (RP) 作为潜在的CacyBP/SIP标,其中RPL6显示了高质量的得分.
  • 生物化学和in silico方法证实了CacyBP/SIP和RPL6之间的直接相互作用,确定了涉及的蛋白质域.
  • 在神经母细胞瘤细胞中,CacyBP/SIP沉默导致新生多的细胞表现出周核染色的显著减少.
  • 与对照细胞相比,CacyBP/SIP沉默细胞在热冲击下显示出明显更高的Hsp70产量,这表明蛋白质合成调节受损.

结论:

  • CacyBP/SIP直接与RPL6和潜在的其他RP相互作用.
  • CacyBP/SIP似乎在核糖体功能和蛋白质合成效率方面发挥作用.
  • 这些发现表明CacyBP / SIP参与细胞蛋白质合成调节,可能是通过与核糖体组件的相互作用.