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编码经典猪瘟病毒E2-ECD的单剂量mRNA疫苗在子中诱导了持久的保护性免疫力
Li-Jun Bian1, Yu Tang1, Fan Yang2,3
1Department of Infectious DiseasesMedical Research InstituteFrontier Science Center for Immunology and Metabolism, Medical Research InstituteTaikang Center for Life and Medical Sciences, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, China.
Veterinary research
|July 21, 2025
概括
针对古典猪瘟病毒 (CSFV) 的新型mRNA疫苗显示出有前途. 这些疫苗在脂质纳米颗粒中封装了E2蛋白,为控制猪瘟疫情提供了潜在的DIVA-compliant战略.
科学领域:
- 兽医病毒学 兽医病毒学
- 疫苗学 疫苗学 疫苗学
- 分子生物学分子生物学
背景情况:
- 经典猪瘟病毒 (CSFV) 在全球范围内造成重大经济损失.
- 目前的活体减弱疫苗存在安全问题,缺乏DIVA能力.
- 需要有效且符合DIVA的疫苗来控制CSFV.
研究的目的:
- 开发和评估脂纳米粒子 (LNP) 封装的mRNA疫苗,编码CSFV E2蛋白.
- 评估这些新型mRNA疫苗的免疫性和保护功效.
- 探索一个符合DIVA的预防古典猪瘟 (CSF) 策略.
主要方法:
- 开发两种编码CSFV E2细胞外域 (E2-ECD) 或其N端片段 (E2-ECD-N) 的LNP-mRNA疫苗.
- 在小鼠中进行免疫学测试,以评估抗原性,抗体反应和T细胞反应.
- 在接种E2-ECD mRNA疫苗后,对子进行保护性免疫力的评估.
主要成果:
- 两种E2-ECD和E2-ECD-NmRNA疫苗都表现出高抗原性,并在单剂量后在小鼠中诱导长期抗体反应.
- 在用mRNA疫苗免疫的小鼠中观察到强大的T细胞反应.
- 一剂E2-ECD mRNA疫苗在子中提供了长达4个月的对CSFV的保护性免疫力.
结论:
- 基于CSFV-E2的mRNA疫苗是预防和控制CSF的一个有希望的策略.
- 这些疫苗有可能符合DIVA标准,有助于疾病管理.
- 开发的mRNA疫苗显示出有效和安全的猪瘟免疫的潜力.
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