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相关概念视频

Clathrin Coated Vesicles01:12

Clathrin Coated Vesicles

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Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
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Introduction to Membrane Traffic01:44

Introduction to Membrane Traffic

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The ER, Golgi apparatus, endosomes, and lysosomes work in tandem to modify, sort, and package proteins and lipids. An integrated membrane trafficking network facilitates the back and forth shuttling of molecules within different organelles in the same cell or across the cell membrane.
The transport of soluble and membrane proteins is mediated by transport vesicles that collect cargo from one cellular compartment and deliver it to another by fusing with the target organelle membrane. The Rab...
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Overview of Secretory Vesicles01:33

Overview of Secretory Vesicles

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Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
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COP Coated Vesicles00:59

COP Coated Vesicles

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Membrane-enclosed structures called vesicles transport proteins and lipids across the cell. The vesicles derive their cargo from the plasma membrane, Golgi, ER, or endosome. Coated vesicles are spherical, protein-coated carriers with a 50–100 nm diameter that mediate bidirectional transport between the ER and the Golgi. The distribution of proteins between the ER and Golgi complex is dynamic and is maintained by different coated vesicles. Their formation is driven by the assembly of...
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Pinching-off of Coated Vesicles01:32

Pinching-off of Coated Vesicles

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Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
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Vesicular Tubular Clusters01:45

Vesicular Tubular Clusters

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After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
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相关实验视频

Updated: Sep 14, 2025

Automating Citrus Budwood Processing for Downstream Pathogen Detection Through Instrument Engineering
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来自类病原体的膜膀中的功能载荷

Gabriel G Araujo1, Matheus M Conforte1, Aline D da Purificação1

  • 1Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, Brazil.

Environmental microbiology reports
|July 22, 2025
PubMed
概括

克桑托蒙纳斯 (Xanthomonas) 香植物有限公司 树产生富含营养和毒性因子的外膜囊泡 (OMV). 这些OMV有助于资源共享和细菌生存,影响类瘤疾病的进展.

关键词:
克桑托蒙纳斯 (Xanthomonas) 是一种埃斯特拉酶的使用方法膜囊泡中的膜泡.蛋白质组学 蛋白质组学

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Small-Scale Plasma Membrane Preparation for the Analysis of Candida albicans Cdr1-mGFPHis
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科学领域:

  • 微生物学 微生物学
  • 植物病理学 植物病理学
  • 细菌病原体的产生

背景情况:

  • 类病是由Xanthomonas citri pv引起的. 这里是Citri.
  • 外膜囊泡 (OMVs) 越来越多地被认为是它们在细菌通信和毒性中的作用.

研究的目的:

  • 研究由Xanthomonas citri pv.产生的OMV的组成和功能. 这里是Citri.
  • 了解OMVs对细菌相互作用和植物殖民的影响.

主要方法:

  • 脂质组分析以比较OMV和全细胞脂质组成.
  • 蛋白质组学分析以识别在OMV中丰富的蛋白质.
  • 基本分析用于量化OMV中的金属.
  • 使用OMV作为唯一的碳来源进行生长测试.
  • 酶活性测定 (酶,蛋白酶).

主要成果:

  • 克桑托蒙纳斯 (Xanthomonas) 香植物有限公司 树产生大量的OMV,有时形成管状结构.
  • 与整个细胞相比,OMVs表现出明显的脂质特征,含有丰富的和心脏脂质.
  • OMVs被丰富了TonB-依赖的受体, siderophores 和必需的金属 (Fe, Zn, Mn).
  • OMVs可以作为细菌生长的碳来源,并具有酶和蛋白酶活动.

结论:

  • 克桑托蒙纳斯 (Xanthomonas) 香植物有限公司 的OMV是资源丰富的囊泡,参与营养共享.
  • 与OMV相关的酶表明在毒性和植物病原发生过程中发挥了作用.
  • 这些发现凸显了OMV在细菌群体相互作用和宿主植物的持久性方面的重要作用.