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相关概念视频

CRISPR01:59

CRISPR

53.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
53.0K
What is Genetic Engineering?00:49

What is Genetic Engineering?

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Overview
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CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

258
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
258
Homologous Recombination02:31

Homologous Recombination

52.3K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
52.3K
Synthetic Biology02:55

Synthetic Biology

5.0K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
5.0K
CRISPR and crRNAs02:53

CRISPR and crRNAs

17.4K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
17.4K

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相关实验视频

Updated: Sep 14, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Genome Editing in Mammalian Cell Lines using CRISPR-Cas

Published on: April 11, 2019

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基因组编辑技术引发的生物安全考虑.

Xinxin Li1, Yuanjiao Gao1, Ziyu Zhang1

  • 1Department of Hepatology Division 2, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China.

Biosafety and health
|July 22, 2025
PubMed
概括

像CRISPR-Cas9这样的基因编辑技术提供了医学进步,但也带来了生物安全风险. 确保安全应用需要通过政策和监管来解决非目标效应,基因组不稳定性和道德问题.

关键词:
生物安全 生物安全聚类有规律间隔的短巴林德罗姆重复关联蛋白9 (CRISPR-Cas9)伦理方面的考虑.基因组编辑技术的技术.监管框架 监管框架 监管框架

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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

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CIRCLE-Seq for Interrogation of Off-Target Gene Editing
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CIRCLE-Seq for Interrogation of Off-Target Gene Editing

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相关实验视频

Last Updated: Sep 14, 2025

Genome Editing in Mammalian Cell Lines using CRISPR-Cas
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Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
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科学领域:

  • 生物技术是生物技术.
  • 遗传学 遗传学 是一个
  • 医学研究 医学研究

背景情况:

  • 脱氧核糖核酸 (DNA) 结构的发现刺激了基因编辑的进步.
  • 基因组编辑技术,特别是CRISPR-Cas9,为生物医学提供了新的机会.

研究的目的:

  • 审查医学中基因编辑的现状.
  • 识别和讨论与基因编辑相关的潜在生物安全风险.
  • 探索确保基因编辑技术的安全和负责任应用的策略.

主要方法:

  • 对基因编辑技术及其应用的文献综述.
  • 对潜在风险的分析,包括非目标效应和基因组不稳定性.
  • 讨论生物安全的政策,监管和技术措施.

主要成果:

  • 基因编辑为生物医学研究和临床应用提供了巨大的潜力.
  • 生物安全问题,包括非目标效应和伦理问题,需要仔细考虑.
  • 临床安全性和疗效需要进一步验证.

结论:

  • 负责任地实施基因编辑需要解决安全,伦理和法律方面的问题.
  • 政策,法规和技术进步对于降低风险至关重要.
  • 为了在医学中安全地推进基因编辑,需要一个全面的视角.