一个乙烯反应因子LllERF092与LllETO1协调,通过激活LllMBF1c在百合花中的热耐受性来提高热耐受性
Jun Xiang1,2, Xue Gong1,2, Qianqian Fang1,2
1Key Laboratory of Landscaping Agriculture/Key Laboratory of Flower Biology and Germplasm Innovation, Ministry of Agriculture and Rural Affairs, College of Horticulture, Nanjing Agricultural University, Nanjing, China.
Plant biotechnology journal
|July 22, 2025
概括
植物的热应激耐受性是由LlERF092/LlETO1-LlMBF1c通路介导的. 这种依赖乙烯的级联通过调节热应激下关键基因表达来增强百合的耐热性.
科学领域:
- 植物生物学 植物生物学
- 分子生物学分子生物学
- 压力生理学 压力生理学
背景情况:
- 多蛋白桥接因子1c (MBF1c) 对植物热应激反应至关重要.
- 在乙烯介导耐热性中,MBF1c的上游调节者尚未完全理解.
研究的目的:
- 在百合花 (Lilium longiflorum) 中识别和描述LlMBF1c的上游调节剂.
- 阐明在热应激下依赖乙烯的耐热性分子机制.
主要方法:
- 酵母单杂交查以确定与LllMBF1c促进体结合的转录因子.
- 定量PCR,核定位分析,以及转基因百合中的过度表达/沉默.
- 免疫沉,然后进行质谱学 (IP-MS) 来识别蛋白相互作用体.
- 乙烯生产试验和外源乙/1-MCP处理.
主要成果:
- LlERF092直接结合并激活了LlMBF1c促进体,在过度表达时增强了耐热性.
- LlETO1与LllERF092相互作用,增强其转录活性并增强耐热性.
- 对LllERF092和LllETO1的联合过度表达比单个过度表达更能产生更大的耐热性.
- 热应激会诱导乙烯的产生,该乙烯由LlERF092/LlETO1-LlMBF1c通路积极调节.
结论:
- LlERF092/LlETO1-LlMBF1c转录级联是花中依赖乙烯的热耐受性的关键调解者.
- 这一途径为植物热应激反应机制提供了新的见解.
- 乙烯信号在增强植物耐热性方面发挥着重要作用.
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