PagHB7/PagABF4-PagEPFL9 模块调节门密度和树的干旱耐受性
Yufei Xia1,2, Ruihua Guo1,2, Te Lu1,2
1State Key Laboratory of Tree Genetics and Breeding, College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, China.
Plant biotechnology journal
|July 22, 2025
概括
家庭盒7 (HB7) 和ABRE结合因子4 (ABF4) 通过准外皮造型因子类9 (EPFL9) 来调节树口腔密度和干旱耐受性. 这揭示了改善植物生长和抗压能力的新遗传资源.
科学领域:
- 植物生物学 植物生物学
- 分子遗传学 分子遗传学
- 植物生理学 植物生理学
背景情况:
- 众所周知,外皮造型因子类9 (EPFL9) 影响树的口腔发育.
- 家庭盒7 (HB7) 和ABRE结合因子4 (ABF4) 在调节口腔密度和干旱耐受性中的作用通过树的EPFL9仍然未被描述.
研究的目的:
- 调查涉及树PagEPFL9,PagHB7和PagABF4的监管网络.
- 阐明这些基因控制口腔发育和干旱耐受性的机制.
主要方法:
- 编辑CRISPR/Cas9基因以创建PageEPFL9突变体和过度表达线.
- 酵母1杂交,酵母2杂交,双分子光补充和双露西法酶记者试验用于研究基因相互作用.
- 电泳运动转移测试和GST拉下测试以确认蛋白质-DNA和蛋白质-蛋白质相互作用.
主要成果:
- 帕格EPFL9定位在护卫细胞中,并对干旱压力做出反应. 在PagEPFL9中功能丧失减少了口腔密度,抑制了生长,并增强了抗旱能力.
- PagHB7 作为 PagEPFL9.9 的上游调节器. 帕格HB7与帕格ABF4相互作用,而帕格ABF4增强了帕格HB7对帕格EPFL9.9的抑制作用.
- 淘汰PagHB7增加了口腔密度,降低了干旱耐受性. 过度表达PagABF4降低了口腔密度,并可能增加干旱耐受性.
结论:
- 帕格HB7和帕格ABF4形成一个相互作用的复合体,通过向树中的帕格EPFL9来调节口腔密度.
- 这种监管途径显著影响植物生长和抗干旱能力,为改善作物提供了宝贵的见解.
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