米中病毒诱导的瘤细胞的分化轨迹通过单细胞RNA测序揭示出来
Nan Wu1, Lu Gan1, Qingqing Suo1
1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Plant biotechnology journal
|July 22, 2025
概括
米黑条纹矮人病毒 (RBSDV) 在米中引起异常细胞发育. 一个新的天文图谱显示,病毒诱导的瘤源于血管膜细胞,具有抑制其生长的特定基因.
科学领域:
- 植物病毒学 植物病毒学
- 分子植物病理学 分子植物病理学
- 单细胞转录组学 单细胞转录组学
背景情况:
- 植物病毒经常诱导异常的宿主细胞分化和发育,导致明显的症状.
- 了解细胞类型对病毒感染的特异反应对于破译植物病毒相互作用至关重要.
研究的目的:
- 通过单细胞RNA测序来研究叶细胞在对米黑色条纹矮人病毒 (RBSDV) 感染的反应中的转录异质性.
- 确定受RBSDV影响的特定细胞类型,并描述病毒诱导的异常细胞发育和瘤形成背后的分子机制.
主要方法:
- 单细胞RNA测序 (scRNA-seq) 在106,973个叶盖细胞中.
- 构建一个单细胞转录组图谱,识别出12种不同的细胞类型.
- 伪素体分析以推断病毒诱导的瘤细胞的发育轨迹.
主要成果:
- 感染RBSDV主要影响体和血管体细胞,导致病毒诱导的瘤形成.
- 瘤细胞分化涉及染色体重塑基因的上调和光合作用和能量代谢基因的下调.
- 一个叶绿体发育基因模块 (DGP1-GLK1-LHCBs) 被抑制,一个与病变发生相关的基因 (JiPR10) 特别抑制瘤增殖.
结论:
- 一个特定于细胞类型的地图揭示了RBSDV通过特定的转录重编程诱导了来自血管膜细胞的瘤形成.
- 与病变发生相关的基因JiPR10通过抑制瘤生长,在宿主耐药性中发挥作用,为疾病管理提供了潜在的目标.
- 这项研究为了解植物对病毒感染和异常发育的反应提供了宝贵的资源.
更多相关视频
13:13Single-cell Quantitation of mRNA and Surface Protein Expression in Simian Immunodeficiency Virus-infected CD4+ T Cells Isolated from Rhesus macaques
Published on: September 25, 2018
10.7K
07:29Characterization of In Vitro Differentiation of Human Primary Keratinocytes by RNA-Seq Analysis
Published on: May 16, 2020
6.2K
相关概念视频
RNA-seq
10.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.4K
Experimental RNAi
6.2K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.2K
Rous Sarcoma Virus (RSV) and Cancer
5.4K
Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
RSV is a retrovirus that contains two copies of a plus-strand RNA genome. Its genome consists of four main open...
5.4K
Viruses with RNA Genomes
114
RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
114
