剧烈耐力运动对骨肌肉替代分离的影响
Alexander Ahn1, Jeongjin J Kim1, Aaron L Slusher1
1School of Kinesiology, University of Michigan, Ann Arbor, Michigan, USA.
bioRxiv : the preprint server for biology
|July 22, 2025
概括
急性耐力运动会改变骨肌肉中的替代拼接 (AS) 和RNA结合蛋白 (RBP) 表达. 长读测序揭示了以前未知的转录多样性和驱动运动适应的机制.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 运动生理学 运动生理学
背景情况:
- 替代拼接 (AS) 是一个关键的转录后过程,通过产生多样化的mRNA变体来扩展蛋白质组.
- 已知急性耐力运动会暂时影响骨肌肉中的AS,但对这些转录组范围内的反应缺乏全面的理解.
研究的目的:
- 为了研究急性耐力运动后骨肌中的差异性AS (DAS) 和差异性异型表达 (DIE).
- 为了比较短读RNA测序 (SRS) 和长读RNA测序 (LRS) 在表征这些运动诱导变化的有效性.
主要方法:
- 从运动研究中分析公开可用的SRS数据.
- 在跑步机炼前后,牛津纳米孔LRS在小鼠胃半腹肌的性能.
- 不同基因表达 (DGE),DAS和DIE分析,使用RT-PCR和免疫血栓进行验证.
主要成果:
- 无论是SRS还是LRS,都在运动后的骨肌肉中发现了显著的DGE,包括89个RNA结合蛋白 (RBP).
- 在SRS分析中,突出显示出外跳转和内保留是常见的AS事件.
- 在LRS分析中,发现了几种具有显著DAS但没有DGE的基因,包括像mSirt2这样的运动相关基因和像mHnrnpa3,mHnrnpa1和mTia1这样的RBP,特别是在运动后24小时 (24pe).
结论:
- 急性耐力运动会诱导骨肌中AS和RBP表达的变化,在24分钟后观察到显著的影响.
- 对于DGE分析,SRS是有效的,但在检测异型水平变化方面存在局限性,可能缺少具有显著DIE的"隐藏"基因.
- LRS提供了一种强有力的方法来揭示新的转录多样性,并阐明耐力练习适应和反应的基础机制.
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