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使用纳米孔DRS和未经修改的转录组控制进行伪乌里丁修饰的差异分析协议

Oleksandra Fanari1, Michele Meseonznik1, Dylan Bloch1

  • 1Department of Bioengineering, Northeastern University, Boston, MA, USA.

STAR protocols
|July 22, 2025
PubMed
概括

本协议详细说明了使用纳米孔直接RNA测序检测信使RNA (mRNA) 中的伪乌里丁修饰的方法. 它分析动态mRNA变化,以应对细胞干扰和蛋白质敲击.

关键词:
生物信息学是一种生物信息学.基因表达 基因表达遗传学 遗传学 是一个基因组学就是基因组学.分子生物学分子生物学在RNA-seqqq.序列分析是指进行序列分析.

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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