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Updated: Sep 14, 2025

Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting
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Robust Comparison of Protein Levels Across Tissues and Throughout Development Using Standardized Quantitative Western Blotting

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使用总蛋白质进行高级正常化,用于人类脂肪细胞的西部斑分析

Leo J S Westerberg1, Benjamin Dedic1, Erik Näslund2

  • 1Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.

PloS one
|July 22, 2025
PubMed
概括

在人类脂肪细胞中,总蛋白质 (TP) 的正常化优于家庭蛋白质. TP显示较低的差异和变异性,确保更可靠的蛋白质表达分析.

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相关概念视频

Western Blotting01:15

Western Blotting

Western blotting is an analytical technique for protein identification. It has various applications in immunology and medicine, including detecting diseases like bovine spongiform encephalopathy, mad cow disease, and human and feline immunodeficiency virus from biological samples.
The technique begins with separating proteins from the sample using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), followed by protein transfer, immunoblotting, and finally, protein detection.
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