相关实验视频

Updated: Sep 14, 2025

Busulfan as a Myelosuppressive Agent for Generating Stable High-level Bone Marrow Chimerism in Mice

Busulfan as a Myelosuppressive Agent for Generating Stable High-level Bone Marrow Chimerism in Mice

Published on: April 1, 2015

布苏尔通过诱导炎来损害精子生成功能

Lingjun Zhao¹, Kaihui Wu¹, Shiyuan Xu^1,2

¹State Key Laboratory of Animal Biotech Breeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences (CAAS), Beijing, China.

|July 22, 2025

概括

布苏尔暴露会通过诱导丸炎症和破坏血液丸屏障,损害男性生殖功能. 这导致精子质量和数量下降,影响生育能力.

科学领域:

生殖毒理学生殖毒理学
细胞生物学细胞生物学
免疫学免疫学免疫学

背景情况:

布苏尔是一种化学疗法剂,已知具有生殖毒性.
布苏尔法因而损害精子生成的确切机制仍然不完全理解.
了解这些机制对于改善不育患者和移植接受者治疗至关重要.

研究的目的:

阐明busulfan对小鼠精子生成功能的损伤机制.
研究布苏尔对丸组织学,精子参数和炎症标志物的影响.
检查布苏尔对血液丸屏障完整性和细胞骨部件的影响.

主要方法:

小鼠接受了布苏尔治疗,并测量了丸和副膜重量.
分析了精子度和形态.
免疫组织化学和免疫光学被用来评估丸组织.
进行了与酶相关的免疫吸收试验 (ELISA),以量化血清炎症因子和与血球屏障相关的蛋白质.

主要成果:

布苏尔法治疗导致丸和副膜重量下降,精子度降低,精子形增加.
组织学分析揭示了丸间歇性细胞透,较小的精卵管和无组织的精子生成细胞.
炎症因子 (IL-6,IL-1β,TNF-α) 的血清水平显著上调.
随着时间的推移,血液丸屏障蛋白 (N-Cadherin,ocludin,connexin 43) 和维丁的水平下降.

结论:

布苏尔会诱发炎,破坏血液丸屏障,并损害精子生成的微环境.
观察到的维门的减少表明细胞骨损伤,损害了Sertoli细胞对精子生成细胞的支持.
这些干扰会对精子生成和雄激素调节产生负面影响.
这些发现为提高精子干细胞移植的安全性和有效性提供了洞察力.

更多相关视频

Microsurgical Obstruction of Testes Fusion in Spodoptera litura

Microsurgical Obstruction of Testes Fusion in Spodoptera litura

Published on: July 16, 2021

Mouse Round Spermatid Injection

Mouse Round Spermatid Injection

Published on: January 26, 2024

相关概念视频

Spermatogenesis

Spermatogenesis

Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...

Infertility in Males

Infertility in Males

Male infertility affects millions of couples worldwide, arising from various factors that impact different stages of the reproductive process. An endocrine imbalance resulting from conditions like hypogonadism, Klinefelter syndrome, or pituitary disorders can disrupt hormone levels and reduce sperm production. Testicular defects, such as tumors, cryptorchidism, atrophic testes, abnormal sperm morphology, and low sperm count or motility, may arise due to genetic factors, structural...

Disorders of the Male Reproductive System

Disorders of the Male Reproductive System

Men's health issues are increasingly recognized as significant, with several conditions posing common threats. Among these, testicular cancer is especially prevalent in younger men, particularly those aged 20 to 35 years. The disease often manifests as a painless mass in the testicles, sometimes accompanied by a sensation of heaviness or a dull ache.
Prostate disorders are another major concern. These conditions can impair urinary flow due to the prostate's location around the urethra....

Testes: Histology

Testes: Histology

A tough, fibrous membrane, the tunica albuginea, covers the testes, extending inward to form fibrous partitions or septa, dividing them into internal compartments called lobules. Each lobule has 1 to 3 tightly coiled seminiferous tubules where sperm production occurs. These tubules merge into a tubular network at the back of the testis, known as the rete testis. It connects to 15 to 20 efferent ductules, leading to the epididymis.
The spermatogenic cells, responsible for producing sperm, are...

Testosterone: Functions and Regulation

Testosterone: Functions and Regulation

The intricate hormonal interplay essential for male reproductive health begins with the release of gonadotropin-releasing hormone (GnRH) by the hypothalamus. This hormone prompts the pituitary gland to secrete follicle-stimulating hormone (FSH) and luteinizing hormone (LH). LH targets the Leydig cells in the testes, stimulating them to produce and release testosterone. In concert with testosterone, FSH acts on the Sertoli cells within the seminiferous tubules to facilitate the release of...

Sexually Transmitted Infections

Sexually Transmitted Infections

Sexually transmitted infections (STIs) are diseases transmitted primarily through unsafe sexual interactions. Bacteria, viruses, or parasites cause them and can result in severe health complications if untreated.ChlamydiaThe bacterium Chlamydia trachomatis is responsible for the disease Chlamydia, the most common STI in the United States. This peculiar pathogen requires human cells to reproduce, residing intracellularly. The initial infection often goes unnoticed because it typically does not...