相关实验视频

Updated: Sep 14, 2025

Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells

Co-Culture of Murine Small Intestine Epithelial Organoids with Innate Lymphoid Cells

Published on: March 23, 2022

微生物失生症塑造了一个系统性的ILC3 / IL-17轴,控制肺炎反应和中央血液形成

Ahmed K Kabil¹, Leo T Liu², Chengxi Xu¹

¹School of Biomedical Engineering, University of British Columbia, Vancouver, BC, Canada.

Mucosal immunology

|July 22, 2025

概括

低剂量的链杆菌素破坏肠道细菌,通过促进Th17/ILC3细胞,增加对肺炎 (过敏性肺炎) 的敏感性. 恢复微生物平衡或抑制mTORC1可以逆转这种效应,突出显示微生物群.

科学领域:

免疫学免疫学免疫学
微生物组研究微生物组研究
宿主-病原体相互作用

背景情况:

疫苗接种和卫生条件的改善与自身免疫和过敏性疾病的增加有关.
主体微生物组的改变被认为是将这些趋势联系在一起的机制.
以前的工作将SCFA产生细菌的枯竭与过敏反应联系在一起.

研究的目的:

为了研究低剂量链杆菌素对免疫细胞群和激活的影响.
确定链杆菌素对Th1/Th17介导疾病,特别是过敏性肺炎 (HP) 的易感性的影响.
阐明将微生物失调与HP联系在一起的潜在机制.

主要方法:

在小鼠中使用低剂量的链杆菌素.
评估肺中的先天性和适应性免疫细胞群和激活状态.
肠道微生物组的枪支元基因组分析.
干预与单离合醇酸,IL-23中和抗体,或mTORC1抑制.

主要成果:

斯特雷普托米辛治疗没有改变过敏反应,但增加了对HP的敏感性.
HP的特征是肺部ILC3s和Th17细胞增加,并激活了APCs.
斯特雷普托米辛诱导的抗生素减少了微生物多样性,减少了胆酸细菌,并丰富了白生物合成途径.
孤立醇酸,IL-23阻断或mTORC1抑制可以逆转HP敏感性和炎症.

结论:

微生物平衡对于确定对过敏性肺炎的易感性至关重要.
产生IL-23的APC和ILC3/Th17细胞之间的反循环将失生症与持续的3型炎症联系起来.
针对这一途径为HP提供了潜在的干预策略.

关键词:

天生的淋巴细胞是先天性淋巴细胞.间歇性肺病间歇性肺病慢性炎症是一种慢性炎症.微生物驱动的血液形成和免疫发育.

更多相关视频

Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment

Characterization of Immune Cells and Proinflammatory Mediators in the Pulmonary Environment

Published on: June 24, 2020

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

Published on: April 21, 2015

相关概念视频

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...

Regulation of Hematopoietic Stem Cells

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...