乙基葡萄在头发中的长期稳定性:通过LC-MS/MS对909个样本进行10年后期分析
Sara Casati1,2, Alessandro Ravelli1, Roberta F Bergamaschi1
1Laboratorio di Tossicologia Forense, Dipartimento di Scienze Biomediche, Chirurgiche ed Odontoiatriche, Università degli Studi di Milano, Milan, Italy.
Drug testing and analysis
|July 22, 2025
概括
头发中的乙基葡萄化物 (EtG) 稳定长达10年,证实了其在法医和公共卫生中长期监测酒精消费的可靠性. 存档的头发样本准确地反映了过去的酒精使用情况.
科学领域:
- 法医毒理学 法医毒理学
- 分析化学 分析化学
- 公共卫生 公共卫生
背景情况:
- 监测长期的酒精消费对于法医和公共卫生至关重要.
- 头发分析乙基葡萄化物 (EtG) 是检测慢性酒精使用的标准方法.
- 在存储的头发样本中EtG的长期稳定性需要全面评估.
研究的目的:
- 评估 EtG 在存储长达 10 年的人类头发中的化学稳定性.
- 为了确定归档的头发样本可靠性,以进行回顾性酒精使用评估.
- 在长期毒理学和法医调查中验证EtG头发分析的使用.
主要方法:
- 重新分析了909个人类头发样本,这些样本最初在2013年至2022年之间进行了测试.
- 使用经过验证的液体染色学-并联质谱法 (LC-MS/MS) 方法进行EtG量化.
- 在储存长达10年的初始和后续分析中EtG度的比较.
主要成果:
- 头发中的EtG度在10年的储存期内没有显著降解.
- 超过80%的样本在重新测试时在分析不确定性范围内产生了匹配的EtG值.
- 一小部分 (4.4%) 的样本在10年后低于慢性酒精使用的30ppg/mg值.
结论:
- 在标准储存条件下,EtG在人类头发中化学稳定至少十年.
- 存档的头发样本可用于评估历史酒精消费.
- EtG在头发中的稳定性加强了其在长期法医和毒理学研究中的有用性.
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