一种验证的定量LC-MS/MS方法,用于在老鼠血中确定deucravacitinib,并将其应用于药理动力学研究
Pottabattula Mahesh1, M Akiful Haque1
1School of Pharmacy, Anurag University, Hyderabad, Telangana, India.
一种新的分析方法在老鼠血中精确量化了deucravacitinib,这是一种TYK2抑制剂,用于斑块性牛皮. 这种经过验证的液体染色学-并联质谱法 (LC-MS/MS) 方法可以进行准确的药理动力学研究.
科学领域:
- 药理学 药理学是指药理学的学科.
- 分析化学 分析化学
- 生物化学 生物化学
背景情况:
- 德乌克拉维西丁是口服的Janus激酶2 (JAK2) 抑制剂,用于治疗中度至重度的斑块性牛皮.
- 准确量化生物矩阵中的药物度对于药理动力学研究至关重要.
研究的目的:
- 开发和验证一种新的分析方法,用于精确量化大鼠血中deucravacitinib.
- 为了使deucravacitinib的敏感和准确的药理学概况.
主要方法:
- 液体染色学-并联质谱学 (LC-MS/MS) 用于检测德乌克拉维西提尼布.
- 一个ACE C18柱和甲醇和氨酸盐的移动相被用于分析分离.
- 方法验证包括对选择性,线性,准确性,精度,恢复性和稳定性的评估.
主要成果:
- 经过验证的LC-MS/MS方法显示出高灵敏度,下限量化值 (LLOQ) 为0.556 ng/mL.
- 从LLOQ到668.132 ng/mL (r2 = 0.9976) 观察到非常好的线性.
- 日内和日间精度分别低于6.62%和5.95%,精度从90.68%到103.80%不等.
结论:
- 开发的LC-MS/MS方法对于在老鼠血中测量deucravacitinib具有强大,选择性和准确性.
- 经过验证的方法适用于deucravacitinib的药理动力学研究.
- 这种方法支持在临床前模型中进一步研究deucravacitinib的药理动力学.
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