底层膜的穿孔引导前后轴形成.
Dong-Yuan Chen1, Nikolas H Claussen2, Shiny Titus3
1Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA, 91125, USA.
Nature communications
|July 22, 2025
概括
不对称的底层膜穿孔引导远部内皮内皮的迁移,在哺乳动物发育中建立前后轴. 这种机制涉及组织重塑和机械力量,在各个物种中都存在.
科学领域:
- 发育生物学 发展生物学
- 细胞迁移 细胞迁移
- 细胞外矩阵生物学 细胞外矩阵生物学
背景情况:
- 前后 (AP) 轴的建立是哺乳动物发育中的一个基本的对称性破坏事件.
- 偏远内脏内皮 (DVE) 的定向迁移对于小鼠的AP轴规范至关重要.
研究的目的:
- 研究底层膜结构在引导DVE迁移中的作用.
- 阐明在哺乳动物发育过程中为DVE迁移建立定向线索的机制.
主要方法:
- 针对地基膜的有针对性的干扰.
- 光板显微镜和组织地图学.
- 物理建模和DVE突起的实时成像.
主要成果:
- 底层膜中的不对称孔径直接导致DVE迁移.
- 矩阵金属蛋白酶产生不均的穿孔,提供方向线索.
- 迁移DVE会使周围组织变形,并且穿孔会在DVE内部编排力生成.
结论:
- 底层膜重塑和机械异质性对于指导定向组织迁移至关重要.
- 在人类和干细胞衍生的胚胎中,前部低质细胞附近的丰富穿孔表明保存的AP轴规范机制.
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